Preliminary genetic characterization of two lineages of black rats (Rattus rattus sensu lato) in Japan, with evidence for introgression at several localities
Preliminary genetic characterization of two lineages of black rats (Rattus rattus sensu lato) in Japan, with evidence for introgression at several localities
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DOI：
10.1266/ggs.80.367
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发表时间：
2005-10-01
影响因子：
1.1
通讯作者：
Suzuki, S
中科院分区：
文献类型：
--
作者：
Chinen, AA;Suzuki, H;Suzuki, S
我们进行了一个试点调查的遗传多样性37核型个体的黑鼠Rattus rattus（广义上）从日本群岛的6个地方，使用线粒体细胞色素B（cyt B）和核内感光视黄酸结合蛋白（IRBP）的完整基因序列。我们的样本包括两个先前记录的核型组：“大洋洲”与2n = 38和“亚洲”与2n = 42。大多数个体的Cyt B序列根据其核型组聚类，组间平均差异为3.8%。一个例外是，从鹿儿岛（九州岛）的个人表现出“亚洲”核型与cyt B单倍型，从“大洋洲”核型组的单倍型不同的单核苷酸取代相结合。共鉴定出6种IRBP单倍型。它们属于三个不同的IRBP谱系（I-III），平均谱系间分歧为1%。在纯合子个体中，这些谱系与核型组表现出良好的关联：IRBP谱系I仅与“大洋洲”核型发生，而IRBP谱系II和III均与“亚洲”核型发生。来自鹿儿岛的个体都具有“亚洲”血统的IRBP，尽管存在“大洋洲”线粒体类型。的Chichijima人口（小笠原群岛）的功能完全“亚洲”核型和cyt B序列，但所有三个IRBP谱系的各种组合。鹿儿岛和Chichijima人口，因此提供了强有力的证据，可行的杂交和两个核型组之间的遗传渐渗，但与变量的遗传结果。我们的结果表明，核型、线粒体和核基因序列的联合分析有可能阐明黑鼠复杂的扩散和种群历史。
We conducted a pilot survey of genetic diversity among 37 karyotyped individuals of the black rat Rattus rattus (sensu lato) from six localities on the Japanese Islands, using complete gene sequences of mitochondrial cytochrome b (cyt b) and nuclear interphotoreceptor retinoid binding protein (IRBP). Our sampling included two previously documented karyotypic groups: 'Oceanian' with 2n = 38 and 'Asian' with 2n = 42. Cyt b sequences for most individuals clustered according to their karyotypic groups, with an average between-group divergence of 3.8%. One exception was that individuals from Kagoshima (Kyushu Island) showed 'Asian' karyotypes combined with a cyt b haplotype that differed by a single nucleotide substitution from the haplotype of the 'Oceanian' karyotypic group. Six IRBP haplotypes were identified. They belonged to three distinct IRBP lineages (I-III), with an average inter-lineage divergence of 1%. Among homozygous individuals, these lineages showed good association with the karyotypic groups: IRBP lineage I occurred only with 'Oceanian' karyotypes, while IRBP lineages II and III both occurred with 'Asian' karyotypes. Individuals from Kagoshima all possessed IRBP of 'Asian' lineages, despite the presence of an 'Oceanian' mitochondrial type. The Chichijima population (Ogasawara Islands) featured exclusively 'Asian' karyotypes and cyt b sequences, but various combinations of all three IRBP lineages. The Kagoshima and Chichijima populations thus provide strong evidence of viable hybridization and genetic introgression between the two karyotypic groups, but with variable genetic outcomes. Our results demonstrate the potential of combined analysis of karyotypes and mitochondrial and nuclear gene sequences to elucidate the complex dispersal and population history of the black rat.