Development of a Golgi-targeted fluorescent chemosensor for detecting ferrous ions overload under Golgi stress
Development of a Golgi-targeted fluorescent chemosensor for detecting ferrous ions overload under Golgi stress
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DOI：
10.1016/j.saa.2023.122560
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发表时间：
2023-03-05
影响因子：
4.4
通讯作者：
He，Longwei
中科院分区：
文献类型：
--
作者：
Li，Ao;Liu，Yalan;He，Longwei
亚铁离子（Fe2+）是体内重要的金属离子，参与氧化和还原相关的疾病。高尔基体是细胞内转运Fe2+的主要亚细胞器，其结构的稳定性与适当浓度的Fe2+有关。本工作合理设计了一种开启型高尔基体靶向荧光化学传感器Gol-Cou-Fe2+，用于灵敏、选择性地检测Fe2+。Gol-Cou-Fe2+在HUVEC和HepG2细胞中表现出优异的检测外源性和内源性Fe2+的能力。它用于捕获缺氧期间上调的 Fe2+ 水平。此外，在高尔基体应激下，结合高尔基体基质蛋白GM130的减少，传感器的荧光随着时间的推移而增强。然而，消除Fe2+或添加一氧化氮(NO)会恢复HUVEC中Gol-Cou-Fe2+的荧光强度和GM130的表达。因此，化学传感器Gol-Cou-Fe2+的发展为追踪高尔基体Fe2+和阐明高尔基体应激相关疾病提供了新的窗口。
Ferrous ion (Fe2+) is a crucial metal ion in the body and participates in the diseases related to oxidation and reduction. Golgi apparatus is the main subcellular organelle of Fe2+transport in cells, and the stability of its structure is related to the Fe2+at an appropriate concentration. In this work, a turn-on type Golgi-targeting fluorescent chemosensorGol-Cou-Fe2+was rationally designed for sensitive and selective detection of Fe2+.Gol-Cou-Fe2+showed excellent capacity of detecting exogenous and endogenous Fe2+in HUVEC and HepG2 cells. It was used to capture the up-regulated Fe2+level during the hypoxia. Moreover, the fluorescence of sensor was enhanced over time under Golgi stress combining with the reduce of Golgi matrix protein GM130. However, elimination of Fe2+or addition of nitric oxide (NO) would restore the fluorescence intensity ofGol-Cou-Fe2+and the expression of GM130 in HUVEC. Thus, development of chemosensorGol-Cou-Fe2+provides a new window for tracking Golgi Fe2+and elucidating Golgi stress-related diseases.