Shotgun Proteomics and Network Analysis Between Plasma Membrane and Extracellular Matrix Proteins From Rat Olfactory Ensheathing Cells
Shotgun Proteomics and Network Analysis Between Plasma Membrane and Extracellular Matrix Proteins From Rat Olfactory Ensheathing Cells
复制标题
大鼠嗅鞘细胞质膜和细胞外基质蛋白之间的鸟枪蛋白质组学和网络分析
DOI：
10.3727/096368910x492607
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发表时间：
2010-01-01
影响因子：
3.3
通讯作者：
Liang, Songping
中科院分区：
文献类型：
--
作者：
Liu, Yisong;Teng, Xiaohua;Liang, Songping
嗅鞘细胞（Olfactory ensheathing cells，OECs）是一类特殊的嗅鞘细胞，具有星形胶质细胞和雪旺细胞的特性。有证据表明，嗅鞘细胞的再生能力是由可溶性，影响其微环境的分泌因子这些因子可以调节OECs自我更新和/或诱导其增强脊髓再生的能力通过高通量表达蛋白质组学方法进行质膜和细胞外基质的分析以鉴定在无血清条件下OECs的质膜和细胞外基质蛋白1D-鸟枪蛋白质组学结合基因本体（GO）分析用于从原代培养的大鼠OECs中筛选蛋白质。鉴定了470种非冗余质膜蛋白和168种细胞外基质蛋白，其中大多数以前从未报道过由OECs产生。根据STRING预测的蛋白质相互作用对质膜蛋白和胞外蛋白进行了分类，定量整合了相互作用数据。在无血清培养条件下，OECs质膜蛋白的蛋白质组学分析及其与分泌组的联系为OECs体内微环境生态位的本质提供了新的见解。
Olfactory ensheathing cells (OECs) are a special type of dial cells that have characteristics of both astrocytes and Schwann cells. Evidence suggests that the regenerative capacity of OECs is induced by soluble, secreted factors that influence their microenvironment These factors may regulate OECs self-renewal and/or induce their capacity to augment spinal cord regeneration Profiling of plasma membrane and extracellular matrix through a high-throughput expression proteomics approach was undertaken to identify plasma membrane and extracellular matrix proteins of OECs under serum-free conditions 1D-shotgun proteomics followed with gene ontology (GO) analysis was used to screen proteins from primary culture rat OECs Four hundred and seventy nonredundant plasma membrane proteins and 168 extracellular matrix proteins were identified, the majority of which were never before reported to be produced by OECs Furthermore. plasma membrane and extracellular proteins were classified based on their protein protein interaction predicted by STRING quantitatively integrates interaction data The proteomic profiling of the OECs plasma membrane proteins and their connection with the secretome in serum-free culture conditions provides new insights into the nature of their in vivo microenvironmental niche Proteomic analysis for the discovery of clinical biomarkers of OECs mechanism warrants further study