Aquimarina celericrescens sp nov., isolated from seawater
Aquimarina celericrescens sp nov., isolated from seawater
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Aquimarina celericrescens sp nov.，从海水中分离
DOI：
10.1099/ijsem.0.002733
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发表时间：
2018
影响因子：
2.8
通讯作者：
Du Zong-Jun
中科院分区：
文献类型：
--
作者：
Wang Nan-Nan;Li Chang-Ming;Li Ying-Xiu;Du Zong-Jun
从中国威海小石岛（37 ° 31 ′ 36 ″ N 122 ° 00 ′ 58 ″ E）近海水域分离到一株橙色、细长杆状、滑行细菌，命名为NS 08T。细胞为革兰氏染色阴性、无孢子形成、无鞭毛、需氧、过氧化氢酶阳性和氧化酶阴性。生长发生在10 - 37 ° C（最佳30 ° C），在1.0 - 5.0%（w/v）NaCl（最佳2.0 - 3.0%）存在下和在pH 6.5 - 9.0（最佳pH 7.0 - 7.5）下。 产生类胡萝卜素色素，但flexirubin型色素没有。主要脂肪酸（> 10%）为异C15：0和异C17：03-OH。 菌株NS 08T的DNA G + C含量为39.4mol%，其唯一的类异戊二烯醌为甲基萘醌MK-6。菌株NS 08T与Aquimarina属模式种Aquimarina muelleri KCTC 12285T的极性脂质组成非常相似，主要成分为磷脂酰乙醇胺（一种未鉴定的氨基脂质）和两种未知的极性脂质。基于16S rRNA基因序列构建的系统发育树显示，NS08T菌株在Aquimarina属内形成了一个进化谱系，与A. JCM 19528T（96.0%）为A级。muelleriKCTC 12285T为95.0%。表型特征将菌株NS 08T与Aquimarina属的描述成员区分开来。根据本研究中提出的证据，菌株NS 08T代表Aquimarina属的一个新种，其名称Aquimarina celericrescenssp. 11月。模式菌株为NS08T（= KCTC 52897T = MCCC 1H00191T）。
An orange-coloured, slender rod-shaped, gliding bacterium, designated NS08T, was isolated from coastal water of Xiaoshi Island, Weihai, China (37° 31′ 36′′ N 122° 00′ 58′′ E). Cells were Gram-stain-negative, non-spore-forming, non-flagellated, aerobic, catalase-positive and oxidase-negative. Growth occurred at 10–37 °C (optimum 30 °C), in the presence of 1.0–5.0 % (w/v) NaCl (optimum 2.0–3.0 %) and at pH 6.5–9.0 (optimum pH 7.0–7.5). Carotenoid pigments were produced but flexirubin-type pigments were not. The major fatty acids (>10 %) were iso-C15 : 0and iso-C17 : 03-OH. The sole isoprenoid quinone of strain NS08Twas menaquinone MK-6 and the DNA G+C content was 39.4 mol%. The polar lipid compositions of strain NS08Tand the type strain of the type species of the genusAquimarina,Aquimarina muelleriKCTC 12285T, were very similar with phosphatidylethanolamine, an unidentified aminolipid and two unknown polar lipids as the major components. A phylogenetic tree based on 16S rRNA gene sequences showed that strain NS08Tformed an evolutionary lineage within the genusAquimarinaand shared the highest level of similarity toA. versatilisJCM 19528T(96.0 %) while level toA. muelleriKCTC 12285Twas 95.0 %. Phenotypic characteristics distinguished strain NS08Tfrom described members of the genusAquimarina. On the basis of the evidence presented in this study, strain NS08Trepresents a novel species of the genusAquimarina, for which the nameAquimarina celericrescenssp. nov. is proposed. The type strain is NS08T(=KCTC 52897T=MCCC 1H00191T).