Novosphingobium tardaugens sp nov., an oestradiol-degrading bacterium isolated from activated sludge of a sewage treatment plant in Tokyo
Novosphingobium tardaugens sp nov., an oestradiol-degrading bacterium isolated from activated sludge of a sewage treatment plant in Tokyo
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DOI：
10.1099/ijs.0.02301-0
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发表时间：
2003-01-01
影响因子：
2.8
通讯作者：
Kikuchi, S
中科院分区：
文献类型：
--
作者：
Fujii, K;Satomi, M;Kikuchi, S
从东京某污水处理厂分离到一株雌二醇降解菌，对其表型、基因分型和系统发育进行了研究。对其16S rDNA序列、DNA碱基组成、全细胞脂肪酸组成和类异戊二烯苯醌组成的分析表明，该分离物为新鞘糖脂属(Novspingobium)。然而，ITS的16S rDNA序列与已知的新天牛属种的序列相似性不超过97%，表明该分离物是独特的。DNA-DNA杂交实验和生理特性鉴定结果也表明，该分离物为一新种，命名为Novphingobium tardauens sp.11月菌株ARI-1(T)(=JCM 11434(T)=ATCC BAA-531(T)=IFO 16725(T))为模式菌株。
An oestradiol-degrading bacterium isolated at a sewage treatment plant in Tokyo was studied phenotypically, genotypically and phylogenetically. Analysis of its 16S rDNA sequence, DNA base composition, whole-cell fatty acid profile and isoprenoid quinone composition, as well as the presence of sphingoglycolipid, revealed that the isolate is a member of the genus Novosphingobium. However, the sequence similarity of its 16S rDNA to those of known Novosphingobium species was no higher than 97 %, implying that the isolate is distinctive. The results of DNA-DNA hybridization experiments and physiological characterization also indicated that the isolate represents a novel Novosphingobium species, for which the name Novosphingobium tardaugens sp. nov. is proposed; strain ARI-1(T) (=jCM 11434(T) =ATCC BAA-531(T) =IFO 16725(T)) is the type strain.