Molecular Diagnosis of Diarrhea: Current Status and Future Potential
Molecular Diagnosis of Diarrhea: Current Status and Future Potential
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DOI：
10.1007/s11908-011-0223-7
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发表时间：
2012-02-01
影响因子：
3.1
通讯作者：
Houpt, Eric R.
中科院分区：
文献类型：
--
作者：
Platts-Mills, James A.;Operario, Darwin J.;Houpt, Eric R.
确定肠道感染的微生物病因仍然是一个难以捉摸的目标。传统的方法，包括文化，显微镜，和抗原为基础的测试有显着的局限性，如检测限和需要多个程序。分子诊断，特别是基于PCR的检测，正在迅速改变传染病的研究和实践。腹泻病具有广泛的潜在感染病因，非常适合多重分子检测。这篇评论强调了目前采用的分子测试的例子，以及这些测试可以在未来应用的方式。腹泻的微生物原因缺乏金标准，这意味着检测到的微生物的临床意义可能并不总是明确的。传统观点认为，应该有一个主要的病原体引起腹泻，但我们的想法受到挑战，越来越多的检测混合感染。因此，成功地将分子诊断学应用于实践，将需要仔细了解技术方面和研究，以确定其临床用途。
Determining the microbiologic etiology of enteric infection remains an elusive goal. Conventional approaches, including culture, microscopy, and antigen-based tests have significant limitations such as limit of detection and the need for multiple procedures. Molecular diagnostics, especially PCR based tests, are rapidly changing research and practice in infectious diseases. Diarrheal disease, with its broad range of potential infectious etiologies, is well suited for multiplex molecular testing. This review highlights examples of currently employed molecular tests, as well as ways in which these tests can be applied in the future. The absence of a gold standard for the microbiologic cause of diarrhea means that the clinical significance of detected organisms may not always be clear. Conventional wisdom is that there should be one main pathogen causing diarrhea, however our thinking is challenged by increased detection of mixed infections. Thus, the successful incorporation of molecular diagnostics for diarrheal disease into practice will require both a careful understanding of the technical aspects and research to define their clinical utility.