Silastic implants for delivery of oestradiol to mice.
Silastic implants for delivery of oestradiol to mice.
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用于向小鼠输送雌二醇的硅橡胶植入物。
DOI：
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
S. Milligan
中科院分区：
文献类型：
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作者：
Paula E. Cohen;S. Milligan
含有雌二醇的硅橡胶植入物被开发出来，用于将一系列生理浓度的雌二醇长期输送到小鼠体内。植入物由离散长度的硅橡胶管组成，其中含有花生油中的雌二醇，在植入物的两端都有一个小的雌二醇溶液储存库。研究表明，雌二醇的体外释放与植入物中类固醇的浓度成正比。含有1到100微克毫升-1的雌二醇的植入物可以在去卵巢的小鼠中引起生物学反应，从对子宫重量和阴道涂片的最小影响到子宫重量的超生理增加和阴道的快速角化。对子宫血管通透性的研究表明，在最初放置植入物的几个小时内就出现了显著的影响。这些结果表明，本研究中所描述的硅橡胶植入物的设计为将受控和易于操作的生理剂量的雌二醇输送到小鼠提供了一种有用的方法。
Silastic implants containing oestradiol were developed for delivering a range of physiological concentrations of oestradiol to mice over long periods. The implants consisted of discrete lengths of Silastic tubing containing oestradiol in arachis oil, with a small reservoir of the oestradiol solution at either end of the implant. Studies showed that the release of oestradiol in vitro was proportional to the concentration of steroid within the implant. Implants containing oestradiol at concentrations from 1 to 100 micrograms ml-1 could induce biological responses in ovariectomized mice, ranging from minimal effects on uterine weight and vaginal smears to supraphysiological increases in uterine weight and rapid vaginal cornification. Studies of uterine vascular permeability indicated that significant effects occurred within a few hours of initial placement of the implant. These results suggest that the design of the Silastic implants described in this study provides a useful method for delivering controlled and easily manipulated physiological doses of oestradiol to mice.
影响因子：
4.8
作者：
BRIDGES, RS
通讯作者：
BRIDGES, RS