Coagulation, Vascular Morphology, and Vasculogenesis in Spinal Ligament Ossification Model Mice
Coagulation, Vascular Morphology, and Vasculogenesis in Spinal Ligament Ossification Model Mice
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DOI：
10.1097/brs.0000000000003891
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发表时间：
2021-08-01
期刊：
影响因子：
3
通讯作者：
Ishibashi，Yasuyuki
中科院分区：
文献类型：
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作者：
Ichikawa，Nana;Kumagai，Gentaro;Ishibashi，Yasuyuki
研究设计：后纵韧带骨化（OPLL）模型小鼠血管系统的体内研究目的：本研究的目的是研究骨化模型中的血液凝固性、血管形态学和血管生成能力，即静脉血栓栓塞（VTE）风险因素，OPLL患者在脊髓损伤后更容易发生静脉血栓栓塞。ttw小鼠脊柱韧带骨化前脊柱韧带毛细血管网浸润。ttw小鼠血管系统的调查可能有助于澄清其pathology.Methods。凝血从ttw和C57 BL/6（WT）小鼠的血液样品进行了评价，在8，16，24周龄。通过测量血管面积，从苏木精-伊红染色切片评估血管形态。从主动脉分离的内皮细胞进行试管形成试验，以评估vasculogenesis.Results.Prothrombin时间显着较短的TTW小鼠比WT在8和16周。16周时，ttw小鼠的纤维蛋白原增加幅度大于WT小鼠。在所有时间点，ttw小鼠的血管面积和血管壁面积均显著小于WT小鼠。在24周时，ttw小鼠的血管壁面积与血管面积的比率显著小于WT小鼠。TTW小鼠的内皮细胞形成的总分支点数量明显高于WT cells.Conclusion.骨化模型小鼠的凝血功能和血管形态受损，血管生成能力强。关于VTE的发病机制，ttw小鼠具有促进VTE发展的环境。证据等级：N/A我们研究了血液凝固性、血管形态和血管生成能力，这些被称为静脉血栓栓塞的危险因素，在后纵韧带骨化模型中，脚尖行走小鼠。足尖行走的小鼠血液凝固和血管环境受损，这可能有助于静脉血栓形成。
Study Design.In vivo studies of the vascular system in ossification of the posterior longitudinal ligament (OPLL) model mice.Objective.The aim of this study was to investigate blood coagulability, vascular morphology, and vasculogenesis capability, known as venous thromboembolism (VTE) risk factors in the ossification model, tiptoe walking (ttw) mice.Summary of Background Data.Patients with OPLL are more likely to develop VTE after spinal cord injury. Capillary mesh invasion of spinal ligaments precedes spinal ligament ossification in ttw mice. Investigation on vascular systems of ttw mice may contribute to clarifying its pathology.Methods.Coagulability of blood samples from ttw and C57BL/6 (WT) mice were evaluated at 8, 16, and 24 weeks of age. Vascular morphology was assessed from a Hematoxylin-Eosin stained section by measuring vessel area. A tube formation assay was performed with endothelial cells isolated from the aorta to assess vasculogenesis.Results.Prothrombin time was significantly shorter in ttw mice than in WT at 8 and 16 weeks. Fibrinogen had a greater increase in ttw mice than in WT at 16 weeks. The vascular area and vascular wall area were significantly smaller in ttw mice than in WT at all timepoints. The ratio of vascular wall area to vascular area was significantly smaller in ttw mice than in WT at 24 weeks. The endothelial cells from ttw mice formed significantly higher numbers of total branching points than WT cells.Conclusion.Ossification model mice had impaired blood coagulation and vascular morphology and high capacity for vasculogenesis. With regard to the pathogenesis of VTE, ttw mice harbor an environment that promotes the development of VTE.Level of Evidence: N/AWe investigated blood coagulability, vascular morphology, and vasculogenesis capability, which are known as venous thromboembolism risk factors, in ossification of the posterior longitudinal ligament model, tiptoe walking mice. The tiptoe walking mice had impaired blood coagulation and vascular environment which may contribute to venous thrombosis.