Hemozoin-generated vapor nanobubbles for transdermal reagent- and needle-free detection of malaria
Hemozoin-generated vapor nanobubbles for transdermal reagent- and needle-free detection of malaria
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DOI：
10.1073/pnas.1316253111
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发表时间：
2014-01-21
影响因子：
11.1
通讯作者：
Lapotko, Dmitri O.
中科院分区：
文献类型：
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作者：
Lukianova-Hleb, Ekaterina Y.;Campbell, Kelly M.;Lapotko, Dmitri O.
疟疾的成功诊断、筛查和消除关键取决于对这种危险感染的快速和灵敏的检测，最好是经皮检测，而不需要复杂的试剂或抽血。目前还没有这种诊断方法。在这里，我们表明，内源性血红素纳米粒子的高吸光度和纳米尺寸称为“疟原虫色素”，一个独特的组成部分，所有血液阶段的疟疾寄生虫，产生一个短暂的蒸汽纳米气泡周围疟原虫色素响应短而安全的近红外皮秒激光脉冲。这些疟疾特异性纳米气泡的声学信号提供了经皮非侵入性和快速检测动物中低至0.00034%的疟疾感染，而无需使用任何试剂或抽血。这些按需瞬时事件在当前疟疾标记物和探针中没有类似物，可以在几秒钟内检测和筛查疟疾，并且可以实现为紧凑、易于使用、廉价和安全的现场技术。
Successful diagnosis, screening, and elimination of malaria critically depend on rapid and sensitive detection of this dangerous infection, preferably transdermally and without sophisticated reagents or blood drawing. Such diagnostic methods are not currently available. Here we show that the high optical absorbance and nanosize of endogenous heme nanoparticles called "hemozoin," a unique component of all blood-stage malaria parasites, generates a transient vapor nanobubble around hemozoin in response to a short and safe near-infrared picosecond laser pulse. The acoustic signals of these malaria-specific nanobubbles provided transdermal noninvasive and rapid detection of a malaria infection as low as 0.00034% in animals without using any reagents or drawing blood. These on-demand transient events have no analogs among current malaria markers and probes, can detect and screen malaria in seconds, and can be realized as a compact, easy-to-use, inexpensive, and safe field technology.