The protective effects of selenium supplementation on ambient PM(2.5-)induced cardiovascular injury in rats
The protective effects of selenium supplementation on ambient PM(2.5-)induced cardiovascular injury in rats
复制标题
补硒对环境PM(2.5-)诱导的大鼠心血管损伤的保护作用
DOI：
10.1007/s11356-018-2292-8
复制
发表时间：
2018
影响因子：
5.8
通讯作者：
Zhao Jinzhuo
中科院分区：
文献类型：
--
作者：
Zeng Xuejiao;Liu Jie;Du Xihao;Zhang Jia;Pan Kun;Shan Wei;Xie Yuquan;Song Weimin;Zhao Jinzhuo
大量的流行病学和实验研究表明，暴露于环境中的细颗粒物(PM2.5)可通过炎症和氧化应激的机制导致人和动物的心肌损伤。本研究旨在探讨补硒酵母(Sey)对PM2.5所致大鼠心血管损伤的预防作用。将56只SD大鼠随机分为7组：生理盐水对照组、溶剂对照组、低、中、高剂量硒预处理组、PM2.5染毒组和高剂量硒对照组。大鼠用不同浓度的SEY处理28d后，隔天气管内滴注PM2.5，共3次。测定各组大鼠左室血和心肌组织中肿瘤坏死因子-α(TN-α)、白介素1-β(IL-1-β)、可溶性细胞间黏附分子-1(sICAM-1)及氧化反应相关指标总抗氧化能力(T-AOC)、总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)水平。结果表明，尽管PM2.5可引起T-AOC、T-AOD和GSH-Px的降低，而MDA和sICM-1的升高，但SEY可引起这些抗氧化指标的剂量依赖性增加和氧化指标的降低。此外，与PM2.5接触组相比，Se预处理组大鼠血清中肿瘤坏死因子-α和IL-1β水平显著降低。结果表明，补硒能有效预防PM2.5所致的心血管炎症和氧化应激反应。结果还表明，营养补充可能是保护人们健康免受空气污染的一种有效方法。
Substantial epidemiological and experimental studies have shown that ambient fine particulate matter (PM2.5) exposure can lead to myocardial damage in human and animal through the mechanism of inflammation and oxidative stress. The purpose of the current study was to investigate whether selenium yeast (SeY) supplementation could prevent cardiovascular injury caused by PM2.5in rats. Fifty-six Sprague-Dawley rats were randomly divided into seven groups: saline control group; solvent control group, low-, middle-, and high-dose Se pretreatment groups, PM2.5exposure group, and high-dose Se control group. The rats were pretreated with different concentration of dietary SeY for 28 days, then were exposed to PM2.5by intratracheal instillation every other day, a total of three times. The levels of inflammatory markers (tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), soluble intercellular adhesion molecule-1 (sICAM-1), and oxidative responses-related indicators total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) were measured in blood and myocardium of the left ventricle. The results showed that although PM2.5caused a decrease of T-AOC, T-AOD, and GSH-Px and increase of MDA and sICM-1, pretreatment with SeY induced a dose-dependent increase in these anti-oxidative indicators and a decrease in oxidative indicators. In addition, the levels of TNF-α and IL-1β in Se pretreatment groups were significantly lower than that in PM2.5exposure group. The results indicated that Se supplementation could effectively prevent cardiovascular inflammation and oxidative stress induced by PM2.5. The results also indicated that the nutritional supplementation might be an effective way to protecting people’s health from air pollution.