Plasma ceruloplasmin as a biomarker for obesity: A proteomic approach
Plasma ceruloplasmin as a biomarker for obesity: A proteomic approach
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DOI：
10.1016/j.clinbiochem.2011.01.014
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发表时间：
2011-04-01
影响因子：
2.8
通讯作者：
Chung, Ji Hyung
中科院分区：
文献类型：
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作者：
Kim, Oh Yoen;Shin, Min-Jeong;Chung, Ji Hyung
目的：本研究旨在利用蛋白质差异显示技术研究肥胖的新生物标志物，特别是与炎症相关蛋白的生物标志物。非肥胖者血浆蛋白质表达的比较（n=109，体重指数，BMI < 25 kg/m2）和肥胖（n=32，BMI >= 25 kg/m（2））组使用二维凝胶电泳（2-DE）分析。结果：在6个差异蛋白质点中，铜蓝蛋白（Cp）和纤维蛋白原在肥胖组中高表达。肥胖组血浆Cp水平显著高于非肥胖组（34.0 +/- 8.6 vs. 41.3 +/- 12.7 mg/dL，p < 0.001），并与年龄（r=0.253，p < 0.005）、BMI（r=0.265，p < 0.001）和hsCRP（r=0.385，p < 0.001）呈正相关。多元逐步回归分析显示，血浆Cp沿着和hsCRP是肥胖的预测因子（校正β系数=0.266，p < 0.01）。(C)2011年加拿大临床化学家协会。爱思唯尔公司出版All rights reserved.
Objectives: This study aimed to investigate new biomarkers of obesity particularly in relation with inflammation-associated proteins using protein differential display techniques.Design and methods: Comparison of protein expression in plasma between non-obese (n=109, body mass index, BMI < 25 kg/m(2)) and obese (n=32, BMI >= 25 kg/m(2)) groups was carried out using two-dimensional gel electrophoresis (2-DE) analysis. ELISA was also performed for validation.Results: Among six differentially expressed protein spots, ceruloplasmin (Cp) and fibrinogen were over-expressed in obese group. Plasma Cp levels were significantly higher in obese group than non-obese group (34.0 +/- 8.6 vs. 41.3 +/- 12.7 mg/dL, p < 0.001) and positively correlated with age (r=0.253, p < 0.005), BMI (r=0.265, p < 0.001) and hsCRP (r=0.385, p < 0.001). In stepwise multiple linear regression analysis, plasma Cp along with hsCRP were found predictors for obesity (adjusted beta-coefficient=0.266, p < 0.01).Conclusion: Elevated plasma Cp levels were significantly associated with obesity, which may be suggested to be a marker of obesity. (C) 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.