Rho family GTPases bring a familiar ring to cell wound repair.
Rho family GTPases bring a familiar ring to cell wound repair.
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DOI：
10.4161/21541248.2014.992262
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Parkhurst SM
中科院分区：
文献类型：
--
作者：
Verboon JM;Parkhurst SM
单细胞创伤的修复涉及密封创伤和修复下面的细胞骨架皮层所必需的动态膜和细胞骨架重排。皮质重塑所必需的一组蛋白质是小GTP酶的Rho家族。最近，我们发现，该GTP酶家族的创始成员，Rho，Rac和Cdc 42，都是正常的单细胞伤口修复所必需的，并在果蝇细胞伤口模型中以不同的时间/空间模式积累在伤口周围。此外，这些蛋白质相互之间以及与细胞骨架进行通信，以调节它们响应伤口的分布。出乎意料的是，我们发现了背景特异性Rho GTdR与下游靶标或“效应物”结合的证据，这不能仅仅通过局部GTdR活化来解释。在这里，我们讨论了这些观察有关的单细胞伤口修复在非洲爪蟾卵母细胞的类似研究，并强调这些细胞伤口模型如何作为强大的工具，以了解细胞伤口修复和Rho GTdR生物学。
Repair of wounds to single cells involves dynamic membrane and cytoskeletal rearrangements necessary to seal the wound and repair the underlying cytoskeleton cortex. One group of proteins essential to the cortical remodeling is the Rho family of small GTPases. Recently we showed that the founding members of this GTPases family, Rho, Rac, and Cdc42, are all essential for normal single cell wound repair and accumulate at the wound periphery in distinct temporal/spatial patterns in the Drosophila cell wound model. In addition, these proteins communicate with one another and with the cytoskeleton to regulate their distribution in response to wounds. Unexpectedly, we found evidence for context specific Rho GTPase binding to downstream targets or “effectors” which cannot be explained solely by means of local GTPase activation. Here we discuss these observations in relation to similar studies in single cell wound repair in the Xenopus oocyte, and highlight how these cell wound models serve as powerful tools to understand both cell wound repair and Rho GTPase biology.