RESPONSES TO LIGHT OF SOLITARY ROD PHOTORECEPTORS ISOLATED FROM TIGER SALAMANDER RETINA
RESPONSES TO LIGHT OF SOLITARY ROD PHOTORECEPTORS ISOLATED FROM TIGER SALAMANDER RETINA
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DOI：
10.1073/pnas.75.7.3507
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发表时间：
1978-01-01
影响因子：
11.1
通讯作者：
SCHWARTZ, EA
中科院分区：
文献类型：
--
作者：
BADER, CR;MACLEISH, PR;SCHWARTZ, EA
用酶法分离虎鳗(Ambystoma Tigrum)视网膜，得到单个分离的杆状感光器。这些孤立细胞保留了完整视网膜杆状细胞的形态特征，并可在培养中保持数天。当刺入微吸管用于电生理记录时，暗适应孤立杆在黑暗中的静息电位约为-45 mV，在静息时的稳态斜率电阻为500 mA。电流-电压关系既有内向整流，也有外向整流。孤立视杆细胞对光的反应与大直径光斑刺激的完整视网膜视杆细胞的反应相似。当测量孤束内段和外段时，其光响应的反转电位均接近O mV。
Single isolated rod photoreceptors were obtained by enzymatic dissociation of the tiger salamander (Ambystoma tigrinum) retina. These solitary cells retained the morphological features of rods of the intact retina and could be maintained in culture for several days. When impaled with micropipettes for electrophysiological recording, dark-adapted solitary rods had during darkness a resting potential of approximatley-45 mV and a steady-state slope resistance of 500 M.OMEGA. at rest. The current-voltage relationship showed both inward- and outward-going rectification. The responses to light of solitary rods were similar to those recorded from rods in the intact retina stimulated with large diameter spots of light. The reversal potential of the light response of solitary rods was near O mV when measured in either the inner or outer segment.