Single-step direct measurement of amyloid fibrils stiffness by peak force quantitative nanomechanical atomic force microscopy
Single-step direct measurement of amyloid fibrils stiffness by peak force quantitative nanomechanical atomic force microscopy
复制标题
DOI：
10.1063/1.3589369
复制
发表时间：
2011-05-09
影响因子：
4
通讯作者：
Mezzenga, Raffaele
中科院分区：
文献类型：
--
作者：
Adamcik, Jozef;Berquand, Alexandre;Mezzenga, Raffaele
我们提出了一个新的原子力显微镜模式称为峰值力攻丝的β-乳球蛋白淀粉样纤维的机械性能的调查的原始应用程序。通过这种技术获得的杨氏模量的值是在完美的协议与通过结合高分子物理和拓扑统计分析原纤维的结构构象获得的原纤维刚度的间接评价。该技术在生物学、软物质和纳米技术中相关的纳米结构物体的弹性性质的估计中显示出很大的希望。(C)2011年美国物理学会。[doi：10.1063/1.3589369]
We present an original application of a new atomic force microscopy mode called peak force tapping for the investigation of the mechanical properties of beta-lactoglobulin amyloid fibrils. The values of Young's modulus obtained by this technique are in perfect agreement with the indirect evaluation of fibrils stiffness obtained by combining polymer physics and topological statistical analysis on fibrils' structural conformations. This technique shows great promise in the estimation of the elastic properties of nanostructured objects relevant in biology, soft matter, and nanotechnology. (C) 2011 American Institute of Physics. [doi:10.1063/1.3589369]