Phenotype of low proliferative potential is dominant in hybrids of normal human fibroblasts
Phenotype of low proliferative potential is dominant in hybrids of normal human fibroblasts
复制标题
正常人成纤维细胞杂交体中低增殖潜力表型占主导地位
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
James R. Smith
中科院分区：
文献类型：
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作者：
O. Pereira;James R. Smith
将具有不同增殖潜能的人二倍体成纤维细胞克隆融合后，用生化选择系统分离杂交种。杂交种的增殖潜力分布与增殖潜力较小的亲本相似。因此，有限分裂的表型在杂交种中占主导地位。这种显性与亲本群体中可以实现七次或更少分裂的细胞有关。在接近其增殖潜力末端的克隆中出现的终末不分裂的细胞不同于那些已经停止分裂(衰老)的克隆中的克隆，因为在与能够分裂的细胞融合后，可以诱导更高比例的细胞至少分裂一次。
A biochemical selection system was used to isolate hybrids after fusion of human diploid fibroblast clones of varying proliferative potential. The distribution of proliferative potentials of the hybrids resembled that of the parent with smaller proliferative potential. Therefore, the phenotype of limited division was dominant in hybrids. This dominance was associated with cells that could achieve seven or less divisions present in the parent populations. Terminally nondividing cells present in clones near the end of their proliferative potential differed from those present in clones that had ceased division (senescent), in that a higher percentage could be induced to divide at least once following fusion with a cell capable of division.