Involvement of antioxidant capacity in quinclorac tolerance in Eleusine indica
Involvement of antioxidant capacity in quinclorac tolerance in Eleusine indica
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DOI：
10.1016/j.envexpbot.2011.05.001
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发表时间：
2011-12-01
影响因子：
5.7
通讯作者：
Matsumoto, Hiroshi
中科院分区：
文献类型：
--
作者：
Sunohara, Yukari;Shirai, Shinjiro;Matsumoto, Hiroshi
为了解牛筋草对二氯喹啉酸（3，7-二氯-8-喹啉羧酸）的耐受机制，研究了牛筋草对活性氧（ROS）的清除能力与其耐受性的关系。indica对除草剂。E.基于处理后6天测定的GR（40）（导致植物生长减少40%所需的除草剂剂量）值，籼稻对二氯喹啉酸的耐受性比马唐高约104倍。二氯喹啉酸（10 μ M）诱导了D.处理后24 h，10 RNA二氯喹啉酸不能诱导E. indica。其固有的超氧阴离子清除活性（SOSA）是E. indica的产量高于D. adscendens。过氧化氢酶（CAT，EC 1.11.1.6）、抗坏血酸过氧化物酶（APX，EC 1.11.1.11）和谷胱甘肽还原酶（GR，EC 1.6.4.2）的组成型活性分别比大肠杆菌高1.9、4.7和3.0倍。indica的产量高于D. adscendens。外源H_2O_2处理降低了E. indica和D. adscendens。而E. indica显著高于D. adscendens，表明E.籼稻具有较强的抗氧化能力，能减轻H_2O_2对植物的毒害作用，且对H_2O_2的耐受性强于籼稻。adscendens。这些结果表明，E.籼稻可能是其对二氯喹啉酸耐受性的一个因素。(C)2011 Elsevier B. V.保留所有权利。
The relationship between quinclorac (3,7-dichloro-8-quinolinecarboxylic acid) tolerance and scavenging capacity of reactive oxygen species (ROS) in Eleusine indica was investigated to understand the tolerance mechanisms of E. indica to the herbicide. E. indica was approximately 104-fold more tolerant to quinclorac than Digitaria adscendens based on GR(40) (herbicide dose required to cause a 40% reduction in plant growth) values determined 6 days after treatment. Quinclorac (10 mu M) induced the overproduction of ROS (presumably superoxide anion (O-2(-))) in the root tips of D. adscendens 24h after treatment. On the other hand, 10 RNA quinclorac did not induce the ROS production in the roots of E. indica. The inherent superoxide anion scavenging activity (SOSA) was 4.2-fold higher in E. indica than that in D. adscendens. The constitutive activities of catalase (CAT, EC 1.11.1.6), ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR. EC 1.6.4.2) were 1.9, 4.7, and 3.0 times higher, respectively in E. indica than those in D. adscendens. Exogenously applied H2O2, one of the main ROS, decreased the chlorophyll content in leaf discs of E. indica and D. adscendens. However, the chlorophyll content in E. indica was much higher than that in D. adscendens, indicating that E. indica with its high antioxidant capacity can alleviate H2O2-induced phytotoxicity and is more tolerant to H2O2 than D. adscendens. These results suggest that the high scavenging capacity of ROS in E. indica could be one factor in its tolerance to quinclorac. (C) 2011 Elsevier B.V. All rights reserved.