Manual methods for sperm motility assessment.
Manual methods for sperm motility assessment.
复制标题
精子活力评估的手动方法。
DOI：
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发表时间：
2013
影响因子：
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通讯作者：
S. Mortimer
中科院分区：
文献类型：
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作者：
D. Mortimer;S. Mortimer
渐进运动是人类精子的一个重要功能特征，它控制着精子穿透和迁移宫颈粘液和卵母细胞的能力，并最终使卵母细胞受精。基于传统的人工/目视方法，提供了一种详细的方案，用于执行准确的四类区分计数，包括可靠地识别快速进展(“a”级)精子--最具生物学意义，因此在临床上最重要的亚群。要达到精度和测量不超过±10%的可接受不确定度，必须经过充分的事先培训，并使用装有加热工作台的显微镜。
Progressive motility is a vital functional characteristic of ejaculated human spermatozoa that governs their ability to penetrate into, and migrate through, both cervical mucus and the oocyte vestments, and ultimately fertilize the oocyte. A detailed protocol, based on traditional manual/visual methods, is provided for performing an accurate four-category differential count including the reliable identification of rapid progressive (grade "a") spermatozoa-the most biologically, and hence clinically, important subpopulation. Thorough prior training and the use of a microscope fitted with a heated stage are both essential requirements for achieving accuracy and an acceptable uncertainty of measurement of no more than ±10%.