OC-2, a novel mammalian member of the ONECUT class of homeodomain transcription factors whose function in liver partially overlaps with that of hepatocyte nuclear factor-6
OC-2, a novel mammalian member of the ONECUT class of homeodomain transcription factors whose function in liver partially overlaps with that of hepatocyte nuclear factor-6
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DOI：
10.1074/jbc.274.5.2665
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发表时间：
1999-01-29
影响因子：
4.8
通讯作者：
Lemaigre, FP
中科院分区：
文献类型：
--
作者：
Jacquemin, P;Lannoy, VJ;Lemaigre, FP
ONECUT类的转录因子，其原型是肝细胞核因子（HNF）-6，其特征在于存在单个切割结构域和特有的同源结构域（Lannoy，V. J.，Burglin，T. R.，卢梭，G. G.，和Lemaigre，F. P.（1998）J. Biol. Chem，273，13552-13562），我们在此报道了人OC-2的鉴定和表征，人OC-2是该类别的第二个哺乳动物成员。OC-2基因位于人类18号染色体上，OC-2 mRNA在人类中的分布是组织限制性的，在肝脏和皮肤中检测到最强的表达。OC-2的氨基酸序列包含几个与HNF-6高度相似的区域。OC-2对肝表达基因调控区中存在的结合位点的识别特性与HNF-6的识别特性不同，但重叠。与HNF-6一样，OC-2在瞬时转染实验中刺激hnf-3 β基因的转录，表明OC-2参与肝脏分化和代谢所需的转录因子网络。
Transcription factors of the ONECUT class, whose prototype is hepatocyte nuclear factor (HNF)-6, are characterized by the presence of a single cut domain and by a peculiar homeodomain (Lannoy, V. J., Burglin, T. R., Rousseau, G. G., and Lemaigre, F. P. (1998) J. Biol. Chem, 273, 13552-13562), We report here the identification and characterization of human OC-2, the second mammalian member of this class. The OC-2 gene is located on human chromosome 18, The distribution of OC-2 mRNA in humans is tissue-restricted, the strongest expression being detected in the liver and skin. The amino acid sequence of OC-2 contains several regions of high similarity to HNF-6, The recognition properties of OC-2 for binding sites present in regulatory regions of liver-expressed genes differ from, but overlap with, those of HNF 6. Like HNF-6, OC-2 stimulates transcription of the hnf-3 beta gene in transient transfection experiments, suggesting that OC-2 participates in the network of transcription factors required for liver differentiation and metabolism.