DNA damage in multiple organs after exposure to chlorhexidine in Wistar rats
DNA damage in multiple organs after exposure to chlorhexidine in Wistar rats
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DOI：
10.1016/j.ijheh.2006.09.001
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发表时间：
2007-03-01
影响因子：
6
通讯作者：
Ribeiro, Daniel A.
中科院分区：
文献类型：
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作者：
Grassi, Tony F.;Camargo, Elaine A.;Ribeiro, Daniel A.
由于氯己定对微生物有效，因此被广泛推荐用于牙科。然而，研究提供的证据表明，洗必泰是有毒的各种类型的细胞。为了确定不同细胞类型中的潜在遗传毒素，本研究的目的是通过单细胞凝胶（彗星）试验研究二葡萄糖酸氯己定是否能够引起白细胞、肝脏、肾脏和膀胱的DNA损伤、改变。将10只雄性Wistar大鼠分为两组：阴性对照组和实验组，实验组用0.12%二葡萄糖酸氯己定3ml灌胃，每天一次，连续8天。如平均尾矩所示，在二葡萄糖酸氯己定处理组的白细胞和肾细胞中观察到DNA损伤统计学显着增加。总之，数据表明，白细胞和肾细胞是口服暴露于氯己定二葡萄糖酸盐后原发性DNA损伤的潜在靶点，如通过单细胞凝胶（彗星）试验检测的。(c)2006年Elsevier GmbH。All rights reserved.
Since chlorhexidine is effective against microorganisms, it is widely recommended in dentistry. However, studies have provided evidence that chlorhexidine is toxic for a variety of cell types. In order to identify potential genotoxins in different cell types, the purpose of this study was to investigate whether chlorhexidine digluconate is able to cause, in terms of DNA damage, alterations in leukocytes, liver, kidney and urinary bladder by the single cell gel (comet) assay. Ten male Wistar rats were divided into two groups: a negative control and the experimental group treated with 3 ml of 0.12% chlorhexidine digluconate by gavage once a day for 8 days. Statistically significant increases of DNA damage was observed in leukocytes and kidney cells of the chlorhexidine digluconate treated group as depicted by the mean tail moment. Taken together, the data indicate that leukocytes and kidney cells are potential targets for primary DNA damage following oral exposure to chlorhexidine digluconate as detected by single cell gel (comet) assay. (c) 2006 Elsevier GmbH. All rights reserved.