A kinetic study of histopathological changes in the subcutis of cats injected with non-adjuvanted and adjuvanted multi-component vaccines
A kinetic study of histopathological changes in the subcutis of cats injected with non-adjuvanted and adjuvanted multi-component vaccines
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DOI：
10.1016/j.vaccine.2007.02.049
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发表时间：
2007-05-16
期刊：
影响因子：
5.5
通讯作者：
Thibault, J.-C.
中科院分区：
文献类型：
--
作者：
Day, M. J.;Schoon, H.-A.;Thibault, J.-C.
本研究的目的是研究猫皮下注射单剂多组分疫苗后的组织反应。三组15只猫被注射了三种疫苗产品中的一种，并以生理盐水作为阴性对照。A组的猫接种了无佐剂疫苗；B组的猫接种了脂类佐剂疫苗；而C组的猫接种了明矾-奎尔A混合物佐剂的产品。分别于接种后第7、21、62天对疫苗和生理盐水注射部位进行采样。对这些疫苗部位的活检进行定性检查，并对与炎症和组织修复反应相关的一系列参数进行半定量评分。对这些数据进行了统计分析，包括主成分分析。在实验的所有三个时间点，与注射非佐剂疫苗相关的炎症显著减少(p=0.000)。虽然所有组在第62天都有组织修复的证据，但那些接受佐剂疫苗的猫有证据表明，在这个较晚的时间点，巨噬细胞内积累了残留的佐剂物质。组织反应的严重程度可能因接种含有佐剂或不含佐剂的疫苗而有很大差异。(C)2007爱思唯尔有限公司。保留所有权利。
The aim of this study was to investigate the subcutaneous tissue response to administration of a single dose of multi-component vaccine in the cat. Three groups of 15 cats were injected with one of three vaccine products with saline as a negative control. Cats in group A received non-adjuvanted vaccine; cats in group B received vaccine with a lipid-based adjuvant; whilst those in group C were vaccinated with a product adjuvanted with an alum-Quil A mixture. The vaccine and saline injection sites were sampled on days 7, 21 and 62 post-vaccination. Biopsies of these vaccine sites were examined qualitatively and scored semi-quantitatively for a series of parameters related to aspects of the inflammatory and tissue repair responses. These data were analysed statistically, including by principal component analysis. At all three time points of the experiment, there was significantly less inflammation associated with administration of non-adjuvanted vaccine (p = 0.000). Although there was evidence of tissue repair by day 62 in all groups, those cats receiving adjuvanted vaccines had evidence of residual adjuvant material accumulated within macrophages at this late time point. The severity of tissue reactions may vary significantly in response to vaccines which include adjuvants or are non-adjuvanted. (C) 2007 Elsevier Ltd. All rights reserved.