Characterizing Variation of Branch Angle and Genome-Wide Association Mapping in Rapeseed (Brassica napus L.).
Characterizing Variation of Branch Angle and Genome-Wide Association Mapping in Rapeseed (Brassica napus L.).
复制标题
油菜（Brassica napus L.）分支角度变异特征和全基因组关联图谱
DOI：
10.3389/fpls.2016.00021
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发表时间：
2016
影响因子：
5.6
通讯作者：
Hu Q
中科院分区：
文献类型：
--
作者：
Liu J;Wang W;Mei D;Wang H;Fu L;Liu D;Li Y;Hu Q
油菜分支角度的变化改变了植物结构，随着种植密度的增加，可以更有效地捕获光。本研究以油菜自然群体为材料，在三种不同的生长环境下，利用60 K Brassica Infinium SNP芯片对油菜分支角性状进行了分析。在20 ~ 70°范围内观察到显著的表型变异。利用TASSEL 4.0中的MLM模型，在A2、A3、A7、C3、C5和C7染色体上共检测到25个与分支角相关的显著数量性状位点。鉴定了参与分支角的功能候选基因的直系同源物。在关键QTL中，A3和C3同源基因组块上的SNPs峰值与关键直链基因BnaA.Lazy1和BnaC.Lazy1接近。除了Lazy（LA）正向同源基因外，拟南芥的鳞状细胞启动子结合蛋白样14（SPL 14）和生长素响应性GRETCHEN哈根3（GH 3）基因被鉴定为靠近A7和C7染色体上的两个SNPs簇。这些发现将有助于进一步了解油菜植株构型，并为油菜分支角度的遗传改良提供参考。
Changes in the rapeseed branch angle alter plant architecture, allowing more efficient light capture as planting density increases. In this study, a natural population of rapeseed was grown in three environments and evaluated for branch angle trait to characterize their phenotypic patterns and genotype with a 60K Brassica Infinium SNP array. Significant phenotypic variation was observed from 20 to 70°. As a result, 25 significant quantitative trait loci (QTL) associated with branch angle were identified on chromosomes A2, A3, A7, C3, C5, and C7 by the MLM model in TASSEL 4.0. Orthologs of the functional candidate genes involved in branch angle were identified. Among the key QTL, the peak SNPs were close to the key orthologous genes BnaA.Lazy1 and BnaC.Lazy1 on A3 and C3 homologous genome blocks. With the exception of Lazy (LA) orthologous genes, SQUMOSA PROMOTER BINDING PROTEIN LIKE 14 (SPL14) and an auxin-responsive GRETCHEN HAGEN 3 (GH3) genes from Arabidopsis thaliana were identified close to two clusters of SNPs on the A7 and C7 chromosomes. These findings on multiple novel loci and candidate genes of branch angle will be useful for further understanding and genetic improvement of plant architecture in rapeseed.