Phenotypic and functional effects of the motheaten gene on murine B and T lymphocytes.
Phenotypic and functional effects of the motheaten gene on murine B and T lymphocytes.
复制标题
motheaten 基因对小鼠 B 和 T 淋巴细胞的表型和功能影响。
DOI：
10.4049/jimmunol.122.3.884
复制
发表时间：
1979
影响因子：
4.4
通讯作者：
T. Chused
中科院分区：
文献类型：
--
作者：
W. Davidson;H. Morse;S. Sharrow;T. Chused
来自C57 BL/6小鼠的me基因纯合子的类肉瘤细胞从早至一周龄就表现出多种表型和功能异常。在B细胞群中，这些包括可检测的表面IG+细胞的频率降低，表面IgM和IgD表达水平的改变，大细胞、浆细胞和TNP特异性空斑形成细胞的频率增加。这些发现一起为B细胞的多克隆活化提供了强有力的证据。me/me脾和淋巴结细胞高水平表达异嗜性MuLV gp 70，为淋巴细胞活化提供了进一步证据。在初步研究中，me/me T细胞似乎在表型和功能上受me基因的影响较小。Thy 1.2在脾脏和淋巴结T细胞表面的分布由低到正常，对ConA和PHA的促有丝分裂反应受到抑制。me/me小鼠的基本缺陷是什么以及它是否主要影响B细胞或所有淋巴细胞仍有待确定。
Lymphoid cells from C57BL/6 mice homozygous for the me gene exhibit multiple phenotypic and functional abnormalities from early as one week of age. In the B cell population these include a reduction in the frequency of detectable surface Ig+ cells, alterations in the level of expression of surface IgM and IgD, an increase in the frequency of large cells, plasma cells and TNP-specific plaque forming cePS. Together these findings provide strong evidence for polyclonal activation of B cells. The high level of expression of xenotropic MuLV gp70 by me/me spleen and lymph node cells provides further evidence for lymphoid cell activation. In preliminary studies, me/me T cells appeared to be phenotypically and functionally less affected by the me gene. The distribution of Thy 1.2 on the surface of spleen and lymph node T cells varied from low to normal and the mitogenic responses to Con A and PHA were depressed. It remains to be determined what the basic deficiency in me/me mice is and whether it affects primarily B cells or all lymphoid cells.