Aphidicolin: an inhibitor of DNA repair in human fibroblasts.
Aphidicolin: an inhibitor of DNA repair in human fibroblasts.
复制标题
Aphidicolin：人类成纤维细胞 DNA 修复抑制剂。
作者：
R. Waters
Aphidicolin是DNA聚合酶α的特异性抑制剂。在254 nm紫外线照射下，研究了其对正常和切除缺陷性着色性干皮病细胞DNA修复的影响。当抑制剂存在时，单链DNA断裂在紫外线照射的正常细胞中积累。在未照射的正常细胞和用该化合物孵育的紫外线照射的切除有效细胞中都没有这种断裂。因此，这些数据表明，aphidicolin阻止了在切除修复过程中形成的单链断裂的重新连接，这意味着DNA聚合酶α参与了人类细胞中DNA的修复。
Aphidicolin is a specific inhibitor of DNA polymerase alpha. Its influence of DNA repair has been studied in both normal and excision deficient xeroderma pigmentosum cells exposed to u.v. irradiation at 254 nm. Single strand DNA breaks accumulated in u.v. irradiated normal cells when the inhibitor was present. Such breaks were absent in both unirradiated normal cells and in u.v. irradiated excision efficient cells incubated with the compound. The data therefore indicate that aphidicolin prevents the rejoining of single strand breaks formed during the excision repair process and imply that DNA polymerase alpha is involved in the repair of DNA in human cells.