ROLE OF AUXIN AND SUCROSE IN THE DIFFERENTIATION OF SIEVE AND TRACHEARY ELEMENTS IN PLANT-TISSUE CULTURES
ROLE OF AUXIN AND SUCROSE IN THE DIFFERENTIATION OF SIEVE AND TRACHEARY ELEMENTS IN PLANT-TISSUE CULTURES
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DOI：
10.1007/bf00390835
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发表时间：
1980-01-01
期刊：
影响因子：
4.3
通讯作者：
ALONI, R
中科院分区：
文献类型：
--
作者：
ALONI, R
研究了胡萝卜愈伤组织中筛管和导管分子的分化，丁香，大豆（Glycine max L.）我的天，向日葵，麻芙蓉和豌豆Pisum sativum L.通过腔隙清除技术，韧皮部的发育先于木质部的发育。在没有愈伤组织中，在没有筛元件的情况下观察到导管元件的分化。以丁香属、胡萝卜属和大豆属的愈伤组织为材料，定量研究了IAA和蔗糖对愈伤组织生长的影响。低IAA水平导致没有导管细胞的筛分子的分化。高水平导致韧皮部和木质部。IAA控制筛和管分子的数量;增加生长素浓度促进这两种细胞类型的数量。蔗糖浓度的变化，而IAA浓度保持不变，并没有一个特定的影响，无论是筛元分化或韧皮部和木质部之间的比例。蔗糖影响沉积在筛板上的胼胝质的量;蔗糖浓度的增加导致胼胝质的量增加。韧皮部可以响应于生长素而形成，而木质部可以响应于生长素以及从韧皮部到达的附加因子而形成。
Differentiation of sieve and tracheary elements was studied in callus culture of Daucus carota L., Syringa vulgaris L., Glycine max (L.) Merr., Helianthus annuus L., Hibiscus cannabinus L. and Pisum sativum L. By the lacmoid clearing technique, development of the phloem commenced before that of the xylem. In no callus was differentiation of tracheary elements observed in the absence of sieve elements. Influence IAA and sucrose was evaluated quantitatively in callus of Syringa, Daucus and Glycine. Low IAA levels resulted in differentiation of sieve elements with no tracheary cells. High levels resulted in both phloem and xylem. IAA controlled the number of sieve and tracheary elements; increase in auxin concentration boosted the number of both cell types. Changes in sucrose concentration while the IAA concentration was kept constant did not have a specific effect on either sieve element differentiation or the ratio between phloem and xylem. Sucrose affected the quantity of callose deposited on the sieve plates; increase in the sucrose concentration resulted in an increase in the amount of callose. Phloem may be formed in response to auxin, while xylem may be formed in response to auxin together with an added factor which is reached from the phloem.