Proteomic Analyses Reveals the Mechanism of Acupotomy Intervention on the Treatment of Knee Osteoarthritis in Rabbits.
Proteomic Analyses Reveals the Mechanism of Acupotomy Intervention on the Treatment of Knee Osteoarthritis in Rabbits.
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DOI：
10.1155/2022/5698387
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发表时间：
2022
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影响因子：
--
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中科院分区：
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--
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针刀介入(AI)是中国治疗膝关节骨性关节炎的一种有效方法，是世界范围内常见的健康问题。然而，人工智能治疗膝关节骨性关节炎的潜在机制仍不清楚。为进一步了解针刀治疗膝骨性关节炎的作用机制，对兔膝关节骨性关节炎模型进行了形态学观察和TMT蛋白质组学分析。通过X线片和核磁共振检查，我们发现人工关节的膝关节间隙大于膝关节骨性关节炎。人工关节软骨细胞排列整齐，膝关节骨性关节炎软骨细胞排列紊乱。通过对软骨细胞的蛋白质组学分析，在AI和KOA中鉴定出68个不同积累的蛋白质(DAP)，并认为与能量代谢和TCA循环有关的DAP在AI的应答中起核心作用。此外，AIFM1被认为是控制线粒体能量产生的重要调节因子。此外，FN1、VIM、COL12A1、COL14A1、MYBPH和DPYSL3在人工智能治疗膝关节骨性关节炎中可能起重要作用。本研究旨在系统阐明针刀干预治疗膝关节骨性关节炎的调节机制。
Acupotomy intervention (AI) is an available treatment for knee osteoarthritis (KOA) in China, which is a common health problem over the world. However, the underlying mechanism of AI on the KOA treatment is still unknown. To further understand the mechanism of acupotomy in treating KOA, the morphological observation and TMT proteomic analyses were conducted in rabbits. By using X-ray and MRI, we found that the space of the knee joint was bigger in AI than in KOA. Moreover, the chondrocytes were neatly arranged in AI but disordered in KOA. With proteomic analyses in chondrocytes, 68 differently accumulated proteins (DAPs) were identified in AI vs. KOA and DAPs related to energy metabolism and the TCA cycle were suggested to play a central role in response to AI. Furthermore, AIFM1 was proposed to be an important regulator in controlling the energy production in mitochondrial. Besides, FN1, VIM, COL12A1, COL14A1, MYBPH, and DPYSL3 were suggested to play crucial roles in AI for the treatment of KOA. Our study was systematically elucidating the regulation mechanism of acupotomy intervention in the treatment of KOA.
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