Arabidopsis genes AS1, AS2, and JAG negatively regulate boundary-specifying genes to promote sepal and petal development
Arabidopsis genes AS1, AS2, and JAG negatively regulate boundary-specifying genes to promote sepal and petal development
复制标题
拟南芥基因 AS1、AS2 和 JAG 负调控边界指定基因以促进萼片和花瓣发育
DOI：
10.1104/pp.107.113787
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发表时间：
2008-02-01
期刊：
影响因子：
7.4
通讯作者：
Huang, Hai
中科院分区：
文献类型：
--
作者：
Ben Xu;Li, Ziyu;Huang, Hai
边界的形成是多细胞真核生物器官发育的关键。在高等植物中，将器官原基与其周围环境分开的边界具有相对较低的细胞增殖率。这种细胞特征受某些边界特异性基因的作用调节，这些基因在器官中的异位表达可导致器官生长抑制。在这里，我们表明，拟南芥不对称叶1和2（AS 1和AS 2）和JAGGED（JAG）基因的功能在萼片和花瓣原基抑制边界指定基因的器官的正常发育。失去功能的as 1 jag和as 2 jag双突变体产生非常小的萼片和花瓣。细胞周期标志物HISTONE 4的分析表明，在萼片原基的双突变体的细胞分裂被抑制。此外，这些异常的萼片和花瓣表现出边界特异性基因PETAL LOSS（PTL）和杯形子叶1和2（CUC 1和CUC 2）的异位过表达。在as 1 jag背景中PTL或CUC 1和CUC 2功能的丧失可以部分挽救微小的萼片和花瓣表型，支持微小的萼片/花瓣表型至少部分由边界指定基因的异位表达引起的模型。总之，我们的数据揭示了一个以前未被认识到的基本调节，AS 1，AS 2和JAG的行为，以确定萼片和花瓣从他们的边界。
Boundary formation is crucial for organ development in multicellular eukaryotes. In higher plants, boundaries that separate the organ primordia from their surroundings have relatively low rates of cell proliferation. This cellular feature is regulated by the actions of certain boundary-specifying genes, whose ectopic expression in organs can cause inhibition of organ growth. Here, we show that the Arabidopsis thaliana ASYMMETRIC LEAVES1 and 2 (AS1 and AS2) and JAGGED (JAG) genes function in the sepal and petal primordia to repress boundary-specifying genes for normal development of the organs. Loss-of-function as1 jag and as2 jag double mutants produced extremely tiny sepals and petals. Analysis of a cell-cycle marker HISTONE4 revealed that cell division in sepal primordia of the double mutant was inhibited. Moreover, these abnormal sepals and petals exhibited ectopic overexpression of the boundary-specifying genes PETAL LOSS (PTL) and CUP-SHAPED COTYLEDONS1 and 2 (CUC1 and CUC2). Loss of PTL or CUC1 and CUC2 functions in the as1 jag background could partially rescue the tiny sepal and petal phenotypes, supporting the model that the tiny sepal/petal phenotypes are caused, at least in part, by ectopic expression of boundary- specifying genes. Together, our data reveal a previously unrecognized fundamental regulation by which AS1, AS2, and JAG act to define sepal and petal from their boundaries.