Effect of Lactobacillus gasseri PA 16/8, Bifidobacterium longum SP 07/3, B-bifidum MF 20/5 on common cold episodes:: A double blind, randomized, controlled trial
Effect of Lactobacillus gasseri PA 16/8, Bifidobacterium longum SP 07/3, B-bifidum MF 20/5 on common cold episodes:: A double blind, randomized, controlled trial
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DOI：
10.1016/j.clnu.2005.02.006
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发表时间：
2005-08-01
影响因子：
6.3
通讯作者：
Schrezenmeir, J
中科院分区：
文献类型：
--
作者：
de Vrese, M;Winkler, P;Schrezenmeir, J
背景和目的：本研究的目的是调查至少 3 个月内服用加氏乳杆菌 PA 16/8、长双歧杆菌 SP 07/3、双歧杆菌 MF 20/5（5 x 10(7) cfu/片）是否会影响症状的严重程度以及普通感冒的发病率和持续时间。方法：随机、双盲、安慰剂对照 干预研究在两个冬春季节进行至少3个月。每天为 479 名健康成年人（18-67 岁）补充维生素和矿物质，无论是否添加益生菌。在补充 14 天之前和之后，在随机抽取的 122 名志愿者亚组中评估了细胞免疫参数。在普通感冒发作期间，参与者每天记录症状。在补充益生菌 14 天之前和之后收集粪便样本，以使用 qRT-PCR 定量粪便乳酸杆菌和双歧杆菌。结果：益生菌治疗组的总症状评分、普通感冒发作持续时间以及发作期间发烧天数均低于对照组：79.3 +/- 7.4 分 vs. 102.5 +/- 12.2 分（P = 0.056)、7.0 +/- 0.5 与 8.9 +/- 1.0 天 (P = 0.045)、0.24 +/- 0.1 与 1.0 +/- 0.3 天 (P = 0.017)。在益生菌治疗组中观察到细胞毒性+ T 抑制细胞 (CD8+) 和 T 辅助细胞 (CD4+) 显着增强。补充益生菌后，粪便中的乳酸杆菌和双歧杆菌显着增加。结论：至少 3 个月内摄入益生菌可显着缩短普通感冒发作时间近 2 天，并减轻症状的严重程度。 (c) 2005 Elsevier Ltd. 保留所有权利。
Background & aims: The aim of this study was to investigate whether the consumption of Lactobacillus gasseri PA 16/8, Bifidobacterium longum SP 07/3, B. bifidum MF 20/5 (5 x 10(7) cfu/tablet) during at least 3 months influences the severity of symptoms and the incidence and duration of the common cold.Methods: A randomized, double-blind, placebo-controlled intervention study was performed over at least 3 months during two winter/spring periods. Four hundred and seventy nine healthy adults (aged 18-67) were supplemented daily with vitamins and minerals with or without the probiotic bacteria. Cellular immune parameters were evaluated in a randomly drawn subgroup of 122 volunteers before and after 14 days of supplementation. During common cold episodes, the participants recorded symptoms daily. Stool samples were collected before and after 14 days of probiotic supplementation to quantify fecal Lactobacilli and Bifidobacteria using qRT-PCR.Results: The total symptom score, the duration of common cold episodes, and days with fever during an episode were lower in the probiotic-treated group than in the control group: 79.3 +/- 7.4 vs. 102.5 +/- 12.2 points (P = 0.056), 7.0 +/- 0.5 vs. 8.9 +/- 1.0 days (P = 0.045), 0.24 +/- 0.1 vs. 1.0 +/- 0.3 days (P = 0.017). A significantly higher enhancement of cytotoxic plus T suppressor cells (CD8+) and a higher enhancement of T helper cells (CD4+) was observed in the probiotic-treated group. Fecal lactobacilli and bifidobacteria increased significantly after probiotic supplementation.Conclusions: The intake of probiotic bacteria during at least 3 months significantly shortened common cold episodes by almost 2 days and reduced the severity of symptoms. (c) 2005 Elsevier Ltd. All rights reserved.