CellCut: A framework for interactive tracking of protein translocations between cell nucleus and cytoplasm
CellCut: A framework for interactive tracking of protein translocations between cell nucleus and cytoplasm
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DOI：
10.1109/isbi.2011.5872782
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发表时间：
2011-03
期刊：
影响因子：
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通讯作者：
Cheng-Jin Du;M. Marcello;D. Spiller;M. White;T. Bretschneider
中科院分区：
文献类型：
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作者：
Cheng-Jin Du;M. Marcello;D. Spiller;M. White;T. Bretschneider
我们提出了一个框架，以相互作用的跟踪蛋白质易位之间的细胞核和细胞质的单细胞。首先，我们使用一种新的交互式分割方法对选定的关键帧进行分割，该方法采用约束密度加权Nyström方法进行特征向量分解，并采用测地线通勤距离进行像素分类。跟踪是通过在细胞核和细胞质边界周围使用一组重叠的子窗口片段从两个关键帧向前和向后传播来实现的。实验表明，该系统能够较好地跟踪复杂的细胞序列。结合有效的验证和手动校正界面，我们的方法将有助于加快长时间序列中多个细胞的并行分析。
We present a framework for interactive tracking of protein translocations between nuclei and cytoplasm of single cells. Initially, we segment selected keyframes using a novel interactive segmentation method, which employs a constrained density weighted Nyström method for eigenvector decomposition, and the geodesic commute distance for pixel classification. Tracking is achieved by both forward and backward propagation from two keyframes using a group of overlapping subwindow segmenters around nuclei and cytoplasm boundaries. Experiments demonstrate that the system is able to track complex cell sequences reasonably well. In combination with efficient interfaces for validation and manual corrections our method will help to speed up the parallel analysis of multiple cells in long time-series.