Characterization of the structure-function relationship at the ligament-to-bone interface
Characterization of the structure-function relationship at the ligament-to-bone interface
复制标题
DOI：
10.1073/pnas.0712150105
复制
发表时间：
2008-06-10
影响因子：
11.1
通讯作者：
Lu, Helen H.
中科院分区：
文献类型：
--
作者：
Moffat, Kristen L.;Sun, Wan-Hsuan S.;Lu, Helen H.
软组织如韧带和肌腱通过分为非钙化和钙化区域的纤维软骨界面与骨结合。不同组织类型之间的这种连接经常受到损伤，并且在手术修复后不能重建。它的再生也受到限制，缺乏了解的结构-功能的内在关系，在这个复杂的接口。因此，关注前交叉韧带（ACL）和骨之间的插入部位，本研究的目的是：（i）确定界面压缩力学性能，（ii）表征界面矿物质的存在和分布，以及（iii）评价机械性能和基质矿物质含量的插入部位依赖性变化。界面的机械性能进行了优化的数字图像相关分析耦合微压缩，而矿物的存在和分布，其特征在于能量色散X射线分析和背散射扫描电子显微镜。两个区域和插入依赖性的机械性能的变化被发现，与钙化界面区域表现出显着更大的压缩力学性能比非钙化区域。矿物质的存在仅在钙化界面和骨区域内可检测到，其分布对应于区域依赖性机械不均匀性。此外，胫骨衬垫的压缩力学性能大于股骨衬垫。本研究阐明的界面结构-功能关系为界面再生和复杂组织系统的形成提供了重要的见解。
Soft tissues such as ligaments and tendons integrate with bone through a fibrocartilaginous interface divided into noncalcified and calcified regions. This junction between distinct tissue types is frequently injured and not reestablished after surgical repair. Its regeneration is also limited by a lack of understanding of the structure-function relationship inherent at this complex interface. Therefore, focusing on the insertion site between the anterior cruciate ligament (ACL) and bone, the objectives of this study are: (i) to determine interface compressive mechanical properties, (ii) to characterize interface mineral presence and distribution, and (iii) to evaluate insertion site-dependent changes in mechanical properties and matrix mineral content. Interface mechanical properties were determined by coupling microcompression with optimized digital image correlation analysis, whereas mineral presence and distribution were characterized by energy dispersive x-ray analysis and backscattered scanning electron microscopy. Both region- and insertion-dependent changes in mechanical properties were found, with the calcified interface region exhibiting significantly greater compressive mechanical properties than the noncalcified region. Mineral presence was only detectable within the calcified interface and bone regions, and its distribution corresponds to region-dependent mechanical inhomogeneity. Additionally, the compressive mechanical properties of the tibial insertion were greater than those of the femoral. The interface structure-function relationship elucidated in this study provides critical insight for interface regeneration and the formation of complex tissue systems.