Soft, porous poly(D,L-lactide-co-glycotide) microcarriers designed for ex vivo studies and for transplantation of adherent cell types including progenitors.
Soft, porous poly(D,L-lactide-co-glycotide) microcarriers designed for ex vivo studies and for transplantation of adherent cell types including progenitors.
复制标题
柔软、多孔的聚（D,L-丙交酯-共-糖苷）微载体，设计用于离体研究和包括祖细胞在内的贴壁细胞类型的移植。
DOI：
10.1111/j.1749-6632.2001.tb03829.x
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发表时间：
2001
影响因子：
5.2
通讯作者：
Reid,LM
中科院分区：
文献类型：
--
作者：
Xu,AS;Reid,LM
摘要：本实验室利用富集的肝祖细胞进行肝脏组织工程。我们在此报告了我们正在进行的研究，设计生物可降解和生物相容性的三维基质支持，天然和合成聚合物材料适合于成体和祖肝细胞的粘附、生长和分化，用于移植，并用于开发生物人工肝辅助装置。以（α‐羟基）酸类聚合物为原料制备了直径为20 ~ 40 μm和100 ~ 300 μm的多孔生物相容性和可生物降解微载体。人类肝癌细胞系HepG2和成年啮齿动物肝细胞被发现附着在胶原包被的聚（d，l -丙交酯- co -糖苷）微载体表面。附着在可降解微载体上的HepG2细胞保持活力并进行生长扩增，在培养中形成三维细胞可降解微载体菌落。这些细胞可降解的微载体菌落可以进行进一步的生长扩增，从而为组织的三维器官发生提供了可行的方法。
Abstract:Our laboratory is undertaking tissue engineering of liver using enriched liver progenitor cells. We report here our ongoing study to design biodegradable and biocompatible three‐dimensional substratum supports of both natural and synthetic polymeric materials suitable to the adhesion, growth, and differentiation of adult and progenitor liver cells for their transplantation, and for the development of a bioartificial liver assist device. Porous biocompatible and biodegradable microcarriers of diameter 20–40 μm and 100–300 μm were prepared from (α‐hydroxy) acid family of polymers. Human hepatoma cell line HepG2 and adult rodent liver cells were found to attach to collagen‐coated surface of poly(d,l‐lactide‐co‐glycotide) microcarriers. HepG2 cells attached to the degradable microcarriers remained viable and underwent growth expansion, forming three‐dimensional cell‐degradable microcarrier colonies in culture. These cell‐degradable microcarrier colonies may undergo further growth expansion, thus providing a viable approach for three‐dimensional organogenesis of tissue.