Monoclonal antibodies isolated from human B cells neutralize a broad range of H1 subtype influenza A viruses including swine-origin Influenza virus (S-OIV)
Monoclonal antibodies isolated from human B cells neutralize a broad range of H1 subtype influenza A viruses including swine-origin Influenza virus (S-OIV)
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DOI：
10.1016/j.virol.2009.12.014
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发表时间：
2010-03-30
期刊：
影响因子：
3.7
通讯作者：
Clementi, Massimo
中科院分区：
文献类型：
--
作者：
Burioni, Roberto;Canducci, Filippo;Clementi, Massimo
新的H1N1猪源流感病毒（S-OIV）毒株是一个全球性的健康问题。阐明病毒-宿主关系对于控制新感染至关重要。在从具有广泛H1N1血清中和活性的患者中出现S-OIV之前，克隆了两种在非常低浓度下中和新型H1N1流感毒株的人单克隆抗体Fab片段（HMab）。这两种HMab中和了所有测试的H1N1毒株，包括S-OIV和一种猪毒株，IC 50范围为2至7 μ g/ml。数据表明，感染以前流行的H1N1病毒株可引发中和S-OIV的抗体。最后，编码中和HMab的人基因可用于产生可安全施用的完整人单克隆IgG，其潜在地可用于预防和治疗这种人感染。(C)2009 Elsevier Inc. All rights reserved.
The new H1N1 swine-origin influenza virus (S-OIV) strain is a global health problem. The elucidation of the virus-host relationship is crucial for the control of the new infection. Two human monoclonal antibody Fab fragments (HMab) neutralizing the novel H1N1 influenza Strain at very low concentrations were cloned before the emergence of S-OIV from a patient who had a broad-range H1N1 serum neutralizing activity. The two HMabs neutralized all tested H1N1 strains, including S-OIV and a swine strain with IC50 ranging from 2 to 7 mu g/ml. Data demonstrate that infection with previously circulating H1N1 strains can elicit antibodies neutralizing S-OIV. Finally, the human genes coding for the neutralizing HMabs could be used for generating full human monoclonal IgGs that can be safely administered being potentially useful in the prophylaxis and the treatment of this human infection. (C) 2009 Elsevier Inc. All rights reserved.