Rapid separation of anionic oligosaccharide species by high performance liquid chromatography.
Rapid separation of anionic oligosaccharide species by high performance liquid chromatography.
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通过高效液相色谱法快速分离阴离子寡糖种类。
DOI：
10.1016/0003-2697(81)90305-5
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发表时间：
1981
影响因子：
2.9
通讯作者：
Natowicz,M
中科院分区：
文献类型：
--
作者：
Baenziger,JU;Natowicz,M
采用MicroPak AX-10离子交换色谱柱(Varian Associates)，流动相为25-500 mm KH_2PO_4，pH为4.0，建立了高效液相色谱快速分离阴离子低聚糖种类的方法。分离含零、一、二、三或四个唾液酸残基的低聚糖只需不到45分钟。该系统还可以分析单酯或双酯键上含有甘露糖-6-PO4部分的低聚糖。在一次层析分析中可以完成多达20毫克的低聚糖的制备性分离，并获得定量的低聚糖产量。该方法可用于阴离子低聚糖的快速分离和表征。
We have developed a method for the rapid separation of anionic oligosaccharide species by high-performance liquid chromatography utilizing a MicroPak AX-10 ion-exchange column (Varian Associates) with the mobile phase consisting of 25–500 mm KH2PO4, pH4.0. Separation of oligosaccharides bearing zero, one, two, three or four sialic acid residues requires less than 45 min. Oligosaccharides containing mannose-6-PO4moieties in monoester or diester linkage can also be analyzed in this system. Preparative separations of as much as 20 mg of oligosaccharide can be accomplished in a single chromatographic analysis with quantitative yields of oligosaccharide. This method should prove useful for the rapid isolation and characterization of anionic oligosaccharide species.