Chemical, elemental, and isotopic effects of acid concentration and treatment duration on ancient bone collagen: an exploratory study
Chemical, elemental, and isotopic effects of acid concentration and treatment duration on ancient bone collagen: an exploratory study
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酸浓度和治疗持续时间对古代骨胶原的化学、元素和同位素影响：一项探索性研究
DOI：
10.1016/j.jas.2010.07.013
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发表时间：
2010
影响因子：
2.8
通讯作者：
W. Pestle
中科院分区：
文献类型：
--
作者：
W. Pestle
尽管关于不同酸浓度或处理时间对胶原蛋白提取物的数量和质量的影响的数据很少，而且盐酸在水解肽键方面极其有效，但最常使用稀盐酸 (HCl) 进行骨脱矿以提取胶原蛋白。对来自史前波多黎各的两块大型古代人类骨骼样本进行多次提取获得的骨胶原产量、元素组成和同位素特征数据表明，0.05 至 0.2 M 浓度范围的酸处理和 24-120 小时的处理之间没有显着差异。因此，使用更浓的酸进行更短的脱矿过程似乎可以在更短的时间内产生高质量的结果。
Demineralization of bone for collagen extraction is most commonly performed using dilute hydrochloric acid (HCl) despite the fact that little data exists on the effects of differing acid concentration or treatment time on the quantity and quality of collagen extracts and that hydrochloric acid is extremely effective at hydrolyzing peptide bonds. Data on yield, elemental composition, and isotopic signature from bone collagen obtained from multiple extractions performed on two large ancient human bone samples from prehistoric Puerto Rico indicate that there is no meaningful difference between acid treatments ranging from 0.05 to 0.2 M concentration and 24–120 h of treatment. As such, a shorter course of demineralization using more concentrated acid would appear to produce quality results in a shorter time.