Glutathione conjugation and induction of a 32,000 dalton stress protein.
Glutathione conjugation and induction of a 32,000 dalton stress protein.
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谷胱甘肽缀合和诱导 32,000 道尔顿应激蛋白。
DOI：
10.1016/0006-2952(89)90040-3
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发表时间：
1989
影响因子：
5.8
通讯作者：
Meredith,MJ
中科院分区：
文献类型：
--
作者：
Freeman,ML;Meredith,MJ
将中国仓鼠卵巢细胞暴露于不同浓度的马来酸二乙酯中，以产生不同程度的细胞内谷胱甘肽(GSH)耗竭。暴露于20μM或更高浓度的马来酸二乙酯可使细胞内谷胱甘肽浓度降低80%或更多，并促进两种32 kDa蛋白质的合成，其等电点约为6.5。细胞暴露于50μ的M丁硫氨酸亚磺胺24小时后，谷胱甘肽水平降低了95%，但不能促进这种蛋白质的合成。然而，在丁硫氨酸亚磺胺处理的细胞中加入马来酸二乙酯可以促进32 kDa蛋白质的合成。暴露于0.4 mM联胺可引发几种热休克蛋白的合成，但不能诱导32 kDa蛋白的合成。这些结果表明，只有在谷胱甘肽耗竭超过80%并需要形成谷胱甘肽结合物时，32 kDa蛋白质的合成才会增强。
Chinese hamster ovary cells were exposed to various concentrations of diethylmaleate in order to produce various levels of intracellular glutathione (GSH) depletion. Exposure to a 20 μM concentration or more of diethylmaleate depleted the intracellular glutathione concentration by 80% or more and resulted in enhanced synthesis of two 32 kDa proteins which exhibited a pI of about 6.5. Exposure of cells to 50 μM buthionine sulfoximine for 24 hr reduced GSH levels by 95% but did not enhance the synthesis of this protein. Addition of diethylmaleate to buthionine sulfoximine-treated cells resulted in enhanced synthesis of the 32 kDa protein however. Exposure to 0.4 mM diamide triggered the synthesis of several heat shock proteins but did not induce the synthesis of the 32 kDa protein. These results indicated that enhanced synthesis of the 32 kDa protein occurred only after glutathione depletion exceeded 80% and required formation of a glutathione conjugate.