Novel patterns of ectopic cell plate growth and lipid body distribution in the Arabidopsis gemini pollen1 mutant
Novel patterns of ectopic cell plate growth and lipid body distribution in the Arabidopsis gemini pollen1 mutant
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DOI：
10.1104/pp.126.2.899
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发表时间：
2001-06-01
期刊：
影响因子：
7.4
通讯作者：
Twell, D
中科院分区：
文献类型：
--
作者：
Park, SK;Twell, D
通过超微结构分析研究了双子花粉1（gem1）突变体配子体细胞异常分裂和花粉细胞命运改变的性质。在gem 1中最早可注意到的缺陷是在早期双细胞阶段出现扩展的膜轮廓。这些细胞被异位的内壁所取代，将细胞质分成两个或多个细胞室。完整或部分内壁是具有高度复杂轮廓的胼胝质，表明引导失败或细胞板生长失调。扩展膜配置文件和延迟胼胝质合成分裂网站进一步提出了一种新的模式的细胞板组装gem 1。gem 1中的多个细胞室在脂体分布方面采用营养细胞的命运。在野生型中，脂质体出现在营养细胞中，而在gem 1中，脂质体积累在所有的细胞质隔室。我们的研究结果支持这一假设，即改变花粉细胞的命运在gem 1的结果异常遗传的细胞命运的决定因素，作为一个结果的干扰胞质分裂。
The nature of aberrant gametophytic cell divisions and altered pollen cell fate in the gemini pollen1 (gem1) mutant was investigated through ultrastructural analysis. The earliest noticeable defect in gem1 was the appearance of extended membrane profiles at the early bicellular stage. These were replaced by ectopic internal walls, which divided the cytoplasm into twin or multiple cell compartments. Complete or partial internal walls were callosic with highly complex profiles, indicating failed guidance or deregulated cell plate growth. Extended membrane profiles and delayed callose synthesis at division sites further suggested a novel pattern of cell plate assembly in gem1. Multiple cell compartments in gem1 adopted vegetative cell fate with regard to lipid body distribution. In the wild type, lipid bodies appear specifically in the vegetative cell, whereas in gem1, lipid bodies accumulated in all cytoplasmic compartments. Our results support the hypothesis that altered pollen cell fate in gem1 results from abnormal inheritance of cell fate determinants as a result of disturbed cytokinesis.