Noninvasive theranostic imaging of HSV-TK/GCV suicide gene therapy in liver cancer by folate-targeted quantum dot-based liposomes
Noninvasive theranostic imaging of HSV-TK/GCV suicide gene therapy in liver cancer by folate-targeted quantum dot-based liposomes
复制标题
基于叶酸靶向量子点的脂质体对肝癌 HSV-TK/GCV 自杀基因治疗的无创治疗诊断成像
DOI：
10.1039/c5bm00077g
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发表时间：
2015-01-01
影响因子：
6.6
通讯作者：
Chen, Li
中科院分区：
文献类型：
--
作者：
Shao, Dan;Li, Jing;Chen, Li
由于纳米技术的发展，治疗诊断学正在成为一种流行的癌症治疗策略。在这项工作中，我们结合了HSV-TK/GCV自杀基因系统和近红外量子点，因为前者在肝癌治疗中非常有效，后者有助于肿瘤成像。将HSV-TK自杀基因与近红外荧光量子点CdSeTe/ZnS核/壳共价偶联，制备了叶酸修饰的治疗诊断脂质体（FL/QD-TK）。对FL/QD-TK进行了体内、外肝癌靶向性研究和生物安全性研究。FL/QD-TK具有高度特异性的肿瘤成像和对叶酸受体过表达的Bel-7402小鼠异种移植物的强烈抑制作用，而无全身毒性。这项研究可能为基因递送和靶向癌症治疗提供线索。
Theranostics is emerging as a popular strategy for cancer therapy; thanks to the development of nano-technology. In this work, we have combined an HSV-TK/GCV suicide gene system and near-infrared quantum dots, as the former is quite effective in liver cancer treatment and the latter facilitates tumor imaging. A folate-modified theranostic liposome (FL/QD-TK) was developed, which is composed of an HSV-TK suicide gene covalently coupling with near-infrared fluorescent CdSeTe/ZnS core/shell quantum dots. The liver cancer-targeting and biosafety of FL/QD-TK were studied in vitro and in vivo. FL/QD-TK exhibited highly specific tumor imaging and strong inhibition of the folate receptor-overexpressed Bel-7402 mouse xenografts without systematic toxicity. This study may shed light on gene delivery and targeted cancer therapy.