Genetic transformation of a pasture legume,Lotuscorniculatus, L. (birdsfoot trefoil)

Genetic transformation of a pasture legume,Lotuscorniculatus, L. (birdsfoot trefoil)
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牧草豆科植物 Lotuscorniculatus, L.(鸟足三叶草)的遗传转化

DOI:
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发表时间:
1989
影响因子:
2.7
通讯作者:
Z. Tabaeizadeh
Z. Tabaeizadeh
中科院分区:
工程技术4区
文献类型:
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作者:
Z. Tabaeizadeh

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摘要以发根农杆菌诱导毛状根为基础,建立了一种外源基因快速导入的方法。在转基因植株中表达了氯霉素乙酰转移酶和新霉素磷酸转移酶II。Southern印迹杂交证实了遗传转化的存在。转基因植株看起来正常,与种子植株相比没有表现出任何形态上的改变。
SummaryA rapid procedure for introducing foreign genes inLotuscorniculatus based on the induction of hairy roots byAgrobacteriumrhizogenes was developed. Expression of chloramphenicol acetyltransferase and neomycin phosphotransferase II was revealed in transgenic plants. Southern blot hybridization was used to confirm the genetic transformation. The transgenic plants looked normal and did not show any morphological modification compared to the seed grown plants.
DOI: 10.1073/pnas.82.17.5824
发表时间: 1985-01-01
影响因子: 11.1
作者:
FROMM, M;TAYLOR, LP;WALBOT, V
通讯作者: WALBOT, V