Seasonal changes in the distribution of black sea bream Acanthopagrus schlegelii estimated by environmental DNA
Seasonal changes in the distribution of black sea bream Acanthopagrus schlegelii estimated by environmental DNA
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通过环境DNA估计黑鲷Acanthopagrus schlegelii分布的季节变化
DOI：
10.1007/s12562-021-01572-z
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发表时间：
2022
影响因子：
1.9
通讯作者：
Masuda Reiji
中科院分区：
文献类型：
--
作者：
Sasano Sachia;Murakami Hiroaki;Suzuki Keita W.;Minamoto Toshifumi;Yamashita Yoh;Masuda Reiji
利用环境DNA技术研究了商业和休闲捕捞的主要目标鱼类--黑鲷的分布特征。12个测量站沿着日本西南部Tango湾的近岸-近海轴线排列。从2017年5月到2018年4月，每月从每个站的表层和底层采集水样（2017年6月采集两次，2017年10月除外）。采用种特异性引物和探针的定量聚合酶链反应（qPCR）技术，对水体中黑鲷DNA进行检测。在这一年中，eDNA在近岸站，特别是在靠近河口的海湾最里面的部分站丰富。在5月和6月，甚至在距离海岸19公里的最离岸站也检测到eDNA，而在7月至4月期间，eDNA仅在距离海岸线3公里的范围内检测到。5月和6月在近海检测到的eDNA可能来自卵和/或幼虫，因为该物种在初夏产卵。这些结果表明，黑鲷在其生活史中对沿海地区有很强的依赖性。
This study aimed at characterizing the distribution of black sea breamAcanthopagrus schlegelii, a major target fish of commercial and recreational fishing, using environmental DNA (eDNA). Twelve survey stations were aligned along an inshore-offshore axis in Tango Bay, southwestern Japan. Water samples were taken from the surface and bottom layers at each station monthly from May 2017 through April 2018 (twice in June 2017 and excluding October 2017). Quantitative polymerase chain reaction (qPCR) with species-specific primers and probe was used to detect DNA of black sea bream in water samples. Throughout the year, eDNA was abundant at inshore stations, especially at stations close to the river mouth in the innermost part of the bay. In May and June, eDNA was detected even at the most offshore station 19 km from the coast, whereas eDNA was detected exclusively within 3 km from the coastline during the months of July through April. The eDNA detected offshore in May and June were likely to have originated from eggs and/or larvae, as this species spawns pelagic eggs in early summer. These results through the use of eDNA indicated that black sea bream is highly dependent on coastal areas in its life history.