Identification and characterization of endosymbiosis-related immune genes in deep-sea musselsGigantidas platifrons
Identification and characterization of endosymbiosis-related immune genes in deep-sea musselsGigantidas platifrons
复制标题
深海贻贝内共生相关免疫基因的鉴定和表征
DOI：
10.1007/s00343-020-0040-7
复制
发表时间：
2020-07-01
影响因子：
1.6
通讯作者：
Li Chaolun
中科院分区：
文献类型：
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作者：
Li Mengna;Chen Hao;Li Chaolun
深海贻贝亚科是广泛分布于冷渗漏和热液喷口的常见的数量优势种。在长期的进化过程中，深海贻贝通过各种方式进化出了很好的适应当地冷渗漏和热液喷口环境的能力，特别是通过与化养细菌建立内共生关系。然而，寄主贻贝和共生体之间建立和维持共生关系的生物学过程在很大程度上是不清楚的。本研究通过脂多糖（LPS）下拉和原位杂交鉴定了可能与甲烷氧化共生体存在共生关系的platfrongigantidas。通过LPS下拉实验，鉴定出toll样受体2 （TLR2）、整合素、液泡分选蛋白（VSP）、基质金属蛋白酶1 （MMP1）、富亮氨酸重复序列（LRR-1）等5种免疫相关蛋白。这五种蛋白在分子序列或功能域上都是保守的，已知是宿主免疫识别、吞噬和溶酶体介导的消化的关键分子。此外，我们还对LRR-1、TLR2和VSP基因进行了原位杂交，研究了它们在鳃组织中的表达模式。platifrons。结果发现，LRR-1、TLR2和VSP基因仅在g的细菌细胞中表达。platifrons。因此，我们认为TLR2、整合素、VSP、MMP1和LRR-1可能是eng之间共生的关键分子。通过参与与共生有关的免疫过程的铂藻和甲烷氧化细菌。
Deep-sea mussels of the subfamily Bathymodiolinae are common and numerically dominant species widely distributed in cold seeps and hydrothermal vents. During long-time evolution, deep-sea mussels have evolved to be well adapted to the local environment of cold seeps and hydrothermal vents by various ways, especially by establishing endosymbiosis with chemotrophic bacteria. However, biological processes underlying the establishment and maintenance of symbiosis between host mussels and symbionts are largely unclear. In the present study,Gigantidas platifronsgenes possibly involved in the symbiosis with methane oxidation symbionts were identified and characterized by Lipopolysaccharide (LPS) pull-down andin situhybridization. Five immune related proteins including Toll-like receptor 2 (TLR2), integrin, vacuolar sorting protein (VSP), matrix metalloproteinase 1 (MMP1), and leucine-rich repeat (LRR-1) were identified by LPS pull-down assay. These five proteins were all conserved in either molecular sequences or functional domains and known to be key molecules in host immune recognition, phagocytosis, and lysosome-mediated digestion. Furthermore,in situhybridization of LRR-1, TLR2 and VSP genes was conducted to investigate their expression patterns in gill tissues ofG. platifrons. Consequently, LRR-1, TLR2, and VSP genes were found expressed exclusively in the bacteriocytes ofG. platifrons. Therefore, it was suggested that TLR2, integrin, VSP, MMP1, and LRR-1 might be crucial molecules in the symbiosis betweenG. platifronsand methane oxidation bacteria by participating in symbiosis-related immune processes.