Analysis of the differential expression profile of miRNAs in myocardial tissues of rats with burn injury
Analysis of the differential expression profile of miRNAs in myocardial tissues of rats with burn injury
复制标题
烧伤大鼠心肌组织中miRNA差异表达谱分析
DOI：
10.1080/09168451.2020.1807901
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发表时间：
2020-08-29
影响因子：
1.6
通讯作者：
Zhu, Xiongxiang
中科院分区：
文献类型：
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作者：
Guo, Jingdong;Zhu, Zhensen;Zhu, Xiongxiang
使用15%三度烧伤大鼠模型来鉴定作为烧伤诱导的心肌损伤的标志物的miRNA。评估心脏组织以确定miRNA谱测序。在miRNAs和损伤标志物之间使用Pearson相关性分析。采用ROC曲线分析方法评价miRNA对烧伤后心肌损伤诊断的敏感性和特异性。测序分析显示23个差异表达的miRNAs。Pearson相关分析显示，rno-miR-190 b-3 p与C5 b 9、rno-miR-341、rno-miR-344 b-3 p与TnI、rno-miR-344 b-3 p与CK-MB分别呈显著正相关。ROC曲线分析表明，rno-miR-341、rno-miR-344 b-3 p和rno-miR-190 b-3 p对烧伤后心肌损伤的诊断具有较高的敏感性和特异性。总之，我们的研究结果表明，rno-miR-341，rno-miR-344 b-3 p和rno-miR-190 b-3 p有可能用作诊断烧伤引起的心肌损伤的敏感和特异性生物标志物。
Fifteen percent third-degree burn rat model was used to identify miRNAs that are markers of burn injury-induced myocardial damage. Cardiac tissues were evaluated to determine miRNA profile sequencing. Pearson's correlation analysis was used between miRNAs and injury markers. ROC curve analysis was used to estimate miRNA's sensitivity and specificity for the diagnosis of myocardial damage caused by burn injury. The sequencing analysis revealed 23 differentially expressed miRNAs. Pearson's correlation analysis revealed that rno-miR-190b-3p and C5b9, rno-miR-341, rno-miR-344b-3p and TnI, rno-miR-344b-3p and CK-MB were significantly positively correlated, respectively. ROC curve analysis demonstrated that rno-miR-341, rno-miR-344b-3p, and rno-miR-190b-3p exhibited high sensitivity and specificity for the diagnosis of myocardial damage caused by burn injury. In conclusion, our results suggest that rno-miR-341, rno-miR-344b-3p, and rno-miR-190b-3p have the potential to be used as sensitive and specific biomarkers to diagnose myocardial damage caused by burn injury.