Single-cell transcriptomics sheds light on the identity and metabolism of developing leaf cells
Single-cell transcriptomics sheds light on the identity and metabolism of developing leaf cells
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DOI：
10.1093/plphys/kiab489
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发表时间：
2022-02-04
期刊：
影响因子：
7.4
通讯作者：
Dubois, Marieke
中科院分区：
文献类型：
--
作者：
Berrio, Ruben Tenorio;Verstaen, Kevin;Dubois, Marieke
在单细胞水平上分析拟南芥幼叶可以定位不同的细胞种群、发育及其对轻度干旱的敏感性。叶片作为维管植物的主要光合作用工具，是至关重要的复杂植物器官。叶肉和表皮细胞层的严格组织协调了光合作用和气体交换。此外，叶片生长所需的水分和营养物质也通过维管组织运输。为了建立这些不同叶片组织的单细胞转录图谱，我们对生长在两种不同环境条件下的拟南芥(Arabiopsis Thaliana)幼苗幼叶分离的单个细胞进行了高通量转录组测序。对1800多个优质叶片细胞中约19,000个不同转录本的检测显示，14个细胞群组成了年轻的、正在分化的叶片。除了组成核心叶组织的细胞种群外，我们还鉴定了具有不同特性或代谢活性的亚种群。此外，我们还提出了针对每个群体的细胞类型特异性标记。最后，一个直观的Web工具允许浏览显示的数据集。我们的数据提供了关于组成发育中的叶片的不同细胞群如何通过发育、代谢或与胁迫相关的轨迹联系在一起的见解。
Profiling young Arabidopsis leaves at the single-cell level enables mapping different cell populations, their development, and their sensitivity to mild drought.As the main photosynthetic instruments of vascular plants, leaves are crucial and complex plant organs. A strict organization of leaf mesophyll and epidermal cell layers orchestrates photosynthesis and gas exchange. In addition, water and nutrients for leaf growth are transported through the vascular tissue. To establish the single-cell transcriptomic landscape of these different leaf tissues, we performed high-throughput transcriptome sequencing of individual cells isolated from young leaves of Arabidopsis (Arabidopsis thaliana) seedlings grown in two different environmental conditions. The detection of approximately 19,000 different transcripts in over 1,800 high-quality leaf cells revealed 14 cell populations composing the young, differentiating leaf. Besides the cell populations comprising the core leaf tissues, we identified subpopulations with a distinct identity or metabolic activity. In addition, we proposed cell-type-specific markers for each of these populations. Finally, an intuitive web tool allows for browsing the presented dataset. Our data present insights on how the different cell populations constituting a developing leaf are connected via developmental, metabolic, or stress-related trajectories.