Molecular detection and genetic diversity of Babesia gibsoni in dogs in Bangladesh.
Molecular detection and genetic diversity of Babesia gibsoni in dogs in Bangladesh.
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DOI：
10.1016/j.meegid.2015.01.011
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发表时间：
2015-04
期刊：
影响因子：
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通讯作者：
M. Terao;S. Akter;Md. Golam Yasin;R. Nakao;H. Kato;M. Alam;K. Katakura
中科院分区：
文献类型：
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作者：
M. Terao;S. Akter;Md. Golam Yasin;R. Nakao;H. Kato;M. Alam;K. Katakura
吉氏巴氏杆菌是一种蜱传的狗的血液原生动物寄生虫，经常引起发烧和溶血性疾病。检测B. Gibsoni主要在亚洲国家报告，包括日本、韩国、台湾、马来西亚、孟加拉国和印度。本研究首次对B.在孟加拉国的狗身上发现了吉布森氏菌。从孟加拉国Mymensingh区的50只流浪狗中采集血样，置于FTA® Elute卡上。从卡片上洗脱的DNA进行巴贝斯虫种18 S rRNA基因的巢式PCR。约800 bp的PCR产物中检测到15 50只狗（30%）。根据限制性片段长度多态性（RFLP）和PCR产物直接测序，所有寄生虫分离物被鉴定为B。吉布森尼。此外，BgTRAP（B.在15份18 S rRNA基因PCR阳性血液样本中，有13份检测到吉布森血小板反应蛋白相关粘附蛋白）基因片段。系统发育分析表明，BgTRAP基因与B.孟加拉国的赤霉病寄生虫形成了一个集群，这是遗传上不同于其他亚洲B。吉布森分离物此外，theBgTRAP基因的串联重复序列分析清楚地表明，孟加拉国分离株之间存在相当大的遗传变异。这些结果表明，B.在孟加拉国，不同遗传分支的吉氏寄生虫在狗中流行。对B.在孟加拉国的狗身上传播的吉布森寄生虫。
Babesia gibsoniis a tick-borne hemoprotozoan parasite of dogs that often causes fever and hemolytic illness. Detection ofB. gibsonihas been predominantly reported in Asian countries, including Japan, Korea, Taiwan, Malaysia, Bangladesh and India. The present study shows the first molecular characterization ofB. gibsonidetected from dogs in Bangladesh. Blood samples were collected on FTA® Elute cards from 50 stray dogs in Mymensingh District in Bangladesh. DNA eluted from the cards was subjected to nested PCR for the 18S rRNA gene ofBabesiaspecies. Approximately 800 bp PCR products were detected in 15 of 50 dogs (30%). Based on restriction fragment length polymorphism (RFLP) and direct sequencing of the PCR products, all parasite isolates were identified asB. gibsoni. Furthermore, theBgTRAP(B. gibsonithrombospondin-related adhesive protein) gene fragments were detected in 13 of 15 18S rRNA gene PCR positive blood samples. Phylogenetic analysis of theBgTRAPgene revealed thatB. gibsoniparasites in Bangladesh formed a cluster, which was genetically different from other AsianB. gibsoniisolates. In addition, tandem repeat analysis of theBgTRAPgene clearly showed considerable genetic variation among Bangladeshi isolates. These results suggested thatB. gibsoniparasites in a different genetic clade are endemic in dogs in Bangladesh. Further studies are required to elucidate the origin, distribution, vector and pathogenesis ofB. gibsoniparasites circulating in dogs in Bangladesh.