IMMUNOCYTOCHEMICAL LOCALIZATION OF A CHONDROITIN SULFATE PROTEOGLYCAN IN NERVOUS-TISSUE .2. STUDIES IN DEVELOPING BRAIN
IMMUNOCYTOCHEMICAL LOCALIZATION OF A CHONDROITIN SULFATE PROTEOGLYCAN IN NERVOUS-TISSUE .2. STUDIES IN DEVELOPING BRAIN
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DOI：
10.1083/jcb.99.3.1130
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发表时间：
1984-01-01
影响因子：
7.8
通讯作者：
MARGOLIS, RK
中科院分区：
文献类型：
--
作者：
AQUINO, DA;MARGOLIS, RU;MARGOLIS, RK
相反，硫酸软骨素蛋白多糖在成人脑中的细胞内（细胞质）定位的免疫电镜研究在未成熟的（出生后7天）大鼠小脑表现出几乎完全细胞外染色的颗粒细胞和分子层。在假定的白色物质区域，染色也是细胞外的和/或与质膜相关。轴突，这是无髓鞘的，在这个年龄，一般不染色，虽然微弱的细胞内染色存在于一些星形胶质细胞。在出生后10和14天有一个显着的减少，细胞外空间和染色，并通过21天的神经元和星形胶质细胞出现明显的胞质染色。这种细胞内染色进一步增加了33天，以密切类似于在成人大脑中看到的模式。从7-d-老和成人脑分离的蛋白多糖的分析表明，它们具有基本相同的生化组成，免疫化学反应，大小，电荷和密度。在这项研究中使用的抗体显然识别相同的大分子在出生后早期和成人的大脑，和本地化的这种蛋白聚糖的变化从细胞外到细胞内的位置在大脑发育过程中进行。
In contrast to the intracellular (cytoplasmic) localization of chondroitin sulfate proteoglycans in adult brain immunoelectron microscopic studies in immature (7 d [days] postnatal) rat cerebellum demonstrated almost exclusively extracellular staining in the granule cell and molecular layers. Staining was also extracellular and/or associated with plasma membranes in the region of the presumptive white matter. Axons, which are unmyelinated at this age, generally did not stain, although faint intracellular staining was present in some astrocytes. At 10 and 14 d postnatally there was a significant decrease in extracellular space and staining, and by 21 d distinct cytoplasmic staining of neurons and astrocytes appeared. This intracellular staining further increased by 33 d so as to closely resemble the pattern seen in adult brain. Analyses of the proteoglycans isolated from 7-d-old and adult brain demonstrated that they have essentially identical biochemical compositions, immunochemical reactivity, size, charge and density. The antibodies used in this study apparently recognize the same macromolecule in both early postnatal and adult brain, and the localization of this proteoglycan changes progressively from an extracellular to an intracellular location during brain development.