AH2 encodes a MYB domain protein that determines hull fate and affects grain yield and quality in rice
AH2 encodes a MYB domain protein that determines hull fate and affects grain yield and quality in rice
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AH2 编码 MYB 结构域蛋白，该蛋白决定稻壳命运并影响水稻的产量和品质
DOI：
10.1111/tpj.14481
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发表时间：
2019-09-03
期刊：
影响因子：
7.2
通讯作者：
Qian, Qian
中科院分区：
文献类型：
--
作者：
Ren, Deyong;Cui, Yuanjiang;Qian, Qian
内稃和外稃（船体）是禾本科植物特有的器官，决定着谷粒的大小和质量。AH 2基因编码一个MYB结构域蛋白，在船体和籽粒的发育过程中发挥重要作用。AH 2突变产生较小的籽粒，并改变籽粒品质，包括直链淀粉含量和胶稠度降低，蛋白质含量增加。同时，部分船体外表面硅化细胞消失，外粗糙表皮细胞向内光滑表皮细胞转化，内稃体缩小。我们通过互补、CRISPR-Cas9以及细胞学和分子检测证实了AH 2的功能。另外，AH 2作为阻遏物，抑制下游基因的转录。我们的研究结果表明，AH 2起着重要的作用，在决定船体表皮发育，内稃身份，和粒度。
The palea and lemma (hull) are grass-specific organs, and determine grain size and quality. In the study, AH2 encodes a MYB domain protein, and functions in the development of hull and grain. Mutation of AH2 produces smaller grains and alters grain quality including decreased amylose content and gel consistency, and increased protein content. Meantime, part of the hull lost the outer silicified cells, and induces a transformation of the outer rough epidermis to inner smooth epidermis cells, and the body of the palea was reduced in the ah2 mutant. We confirmed the function of AH2 by complementation, CRISPR-Cas9, and cytological and molecular tests. Additionally, AH2, as a repressor, repress transcription of the downstream genes. Our results revealed that AH2 plays an important role in the determination of hull epidermis development, palea identity, and grain size.