trans,trans-Muconic acid, an open-chain urinary metabolite of benzene in mice. Quantification by high-pressure liquid chromatography.
trans,trans-Muconic acid, an open-chain urinary metabolite of benzene in mice. Quantification by high-pressure liquid chromatography.
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反式，反式粘康酸，小鼠体内苯的开链尿代谢物。
DOI：
10.3109/00498258509045351
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
M. Legator
中科院分区：
文献类型：
--
作者：
M. M. Gad;V. Ramanujam;M. Legator
一个敏感的H.P.L.C.描述了从苯处理的雄性CD-1小鼠中分离尿代谢物的方法。48h尿中苯酚、反式、反式肉豆蔻酸和对苯二酚分别占单次口服苯染毒(880、440和220 mg/kg体重)的12.8~22.8、1.8~4.7和1.5~3.7%。邻苯二酚以微量形式存在。用抗坏血酸调节尿液pH值，提高代谢物，特别是麦芽酸的提取效率。它通过防止对苯二酚的自动氧化，对其进行了准确的估计。在口服苯酚、邻苯二酚和对苯二酚(分别为250、150和200 mg/kg)的小鼠的尿液中未检测到反式，反式-麦角酸，这是苯所特有的。讨论了一种有毒的苯代谢物在体内的潜在存在，该代谢物是肉豆蔻酸的醛前体。
A sensitive h.p.l.c. method is described which separated urinary metabolites from benzene-treated male CD-1 mice. Phenol, trans,trans-muconic acid and quinol in the 48 h urine accounted, respectively, for 12.8-22.8, 1.8-4.7 and 1.5-3.7% of the orally administered single dose of benzene (880, 440 and 220 mg/kg body wt.). Catechol occurred in trace amounts. Ascorbic acid was used to adjust urine pH and increase the extraction efficiency of metabolites, especially muconic acid. It allowed an accurate estimation of quinol by preventing its auto-oxidation. trans,trans-Muconic acid was identified and was unique to benzene as none was detected in urine of mice dosed orally with phenol, catechol or quinol (250, 150 and 200 mg/kg, respectively). The potential existence of a toxic benzene metabolite in the form of an aldehyde precursor of muconic acid in vivo is discussed.