In vivo osteogenic response to different ratios of BMP-2 and VEGF released from a biodegradable porous system
In vivo osteogenic response to different ratios of BMP-2 and VEGF released from a biodegradable porous system
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DOI：
10.1002/jbm.a.34183
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发表时间：
2012-09-01
影响因子：
4.9
通讯作者：
Evora, Carmen
中科院分区：
文献类型：
--
作者：
Hernandez, Antonio;Reyes, Ricardo;Evora, Carmen
以兔股骨髓内缺损区为模型，研究了在PLGA微球中负载血管内皮生长因子(0.35和1.75mU g)、骨形态发生蛋白-2(3.5mU/g和17.5mgBMP-2)及BMP-2和小剂量血管内皮生长因子(VEGF)复合后的骨再生和血管生成情况。该系统旨在控制生长因子(GF)的释放，并保持GFS定位于缺陷内。体外释放不完全，而体内VEGF和BMP-2分别在3周和4周内完全释放。在4周时，血管内皮生长因子和骨形态发生蛋白-2双重给药(大剂量)的协同作用较弱。然而，与BMP-2相比，联合用药缺乏明显的长期协同效应(12周)，这表明需要做更多的工作来优化VEGF的剂量、顺序呈现和两种GFS的比例，以获得有益的骨修复反应。(C)2012年威利期刊公司《生物材料研究》A部分：100A：23822391,2012年。
Bone regeneration and vascularization with porous PLGA scaffolds loaded with VEGF (0.35 and 1.75 mu g) and BMP-2 (3.5 and 17.5 mu g), incorporated in PLGA microspheres, or the combination of either dose of BMP-2 with the low dose of VEGF were investigated in an intramedullary femur defect in rabbits. The system was designed to control growth factor (GF) release and maintain the GFs localized within the defect. An incomplete release was observed in vitro whereas in vivo VEGF and BMP-2 were totally delivered during 3 and 4 weeks, respectively. A weak synergistic effect of the dual delivery of VEGF and BMP-2 (high dose) was found by 4 weeks. However, the absence of an apparent synergistic long-term effect (12 weeks) of the combination over BMP-2 alone suggests that more work has to be done to optimize VEGF dose, sequential presentation, and the ratio of the two GFs to obtain a beneficial bone repair response. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A: 23822391, 2012.