Application of serum pharmacology in evaluating the antitumor effect of Fuzheng Yiliu Decoction (aeparts per thousandae£aeSc∼currency signae-1) from Chinese medicine
Application of serum pharmacology in evaluating the antitumor effect of Fuzheng Yiliu Decoction (aeparts per thousandae£aeSc∼currency signae-1) from Chinese medicine
复制标题
DOI：
10.1007/s11655-013-1544-1
复制
发表时间：
2014-06-01
影响因子：
2.9
通讯作者：
Du Jian
中科院分区：
文献类型：
--
作者：
Chen Xu-zheng;Cao Zhi-yun;Du Jian
探讨血清药理学评价扶正固本中药抗肿瘤作用的可行性（千分之一ae一磅>（0）aeenot标志，支持健康的能量和增强身体的抵抗力病原体），扶正抑瘤汤的影响（ae parts per thousand ae ae ae Sc磅similar to currency signae-（1），FYD），扶正固本的典型方剂，分别采用中药血清药理学和传统药理学两种方法观察FYD含药血清和FYD粗提物对体外培养的肝癌细胞HepG 2增殖和凋亡的影响。MTT法测定细胞增殖率。流式细胞仪检测细胞周期和凋亡率。用放射免疫法检测扶正固本中药抗肿瘤血清中白细胞介素2（IL-2）和肿瘤坏死因子α（TNF-α）的含量，结果与我们前期动物实验和临床实验结果一致，表明血清药理学方法是评价扶正固本中药抗肿瘤作用的合理可行的方法。
To investigate the feasibility of serum pharmacology in evaluating the antitumor effect of Chinese medicine (CM) of Fuzheng Guben (ae parts per thousand ae a pound >(0)aeenot sign, supporting the healthy energy and strengthening the body's resistance to pathogens), the effects of Fuzheng Yiliu Decoction (ae parts per thousand ae aeSc pound similar to currency signae-(1), FYD), a typical prescription of Fuzheng Guben, on proliferation and apoptosis of hepatoma cells in vitro were observed by two methods with serum pharmacology and traditional pharmacology, respectively.HepG2 cells were treated with FYD-containing serum or crude FYD extract in vitro. The proliferation rate was determined by methyl thiazolyl tetrazolium (MTT) assay. Cell cycle and apoptosis rate was performed by flow cytometry. And the levels of interleukin-2 (IL-2) and tumor necrosis factor alpha (TNF-alpha) in FYD-containing serum were detected by radioimmunoassay.The results by serum pharmacology were accordant with those of our previous animal and clinical trials which indicates that serum pharmacology is a reasonable and feasible method for the evaluation of the antitumor effect of herbs of Fuzheng Guben.