Use of serologic tests to predict resistance to feline herpesvirus 1, feline calicivirus, and feline parvovirus infection in cats
Use of serologic tests to predict resistance to feline herpesvirus 1, feline calicivirus, and feline parvovirus infection in cats
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DOI：
10.2460/javma.2002.220.38
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发表时间：
2002-01-01
期刊：
影响因子：
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通讯作者：
Jensen, WA
中科院分区：
文献类型：
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作者：
Lappin, MR;Andrews, J;Jensen, WA
目的：确定血清病毒特异性抗体的检测是否与猫对强毒猫疱疹病毒I型(FHV-1)、猫杯状病毒(FCV)和猫细小病毒(FPV)攻击的抵抗力相关，并确定客户拥有的猫的血清FHV-1、FCV和FPV抗体的百分比。设计前瞻性实验研究。动物-72只实验室饲养的猫和276只客户拥有的猫。程序-实验室饲养的猫接种了FHV-1、FCV和FPV疫苗，或作为未接种的对照。在接种疫苗后的9到36个月之间，猫受到了强毒病毒的攻击。建立了检测FHV-1、FCV和FHV特异性抗体的重组抗原ELISA，并将检测结果与血凝抑制(FPV)和病毒中和试验(FHV-1和FCV)以及对病毒攻击的抵抗力进行了比较。结果：对于接种疫苗的实验室饲养的猫，FHV和FCV的阳性预测值为100%，FHV-1的阳性预测值为90%。在客户拥有的276只猫中，FHV-1、FCV和FPV的检测结果分别为195只(70.7%)、255只(92.4%)和189只(68.5%)。结论和临床相关性-结果表明，对于接种过疫苗的猫，检测FHV-1、FCV和FPV特异性抗体可以预测猫是否易患疾病，无论疫苗类型或接种间隔。因为大多数客户拥有的猫都有可检测到的血清抗体，表明对感染的抵抗力，使用任意的加强疫苗接种间隔可能会导致一些猫接种不必要的疫苗。
Objective-To determine whether detection of virus-specific serum antibodies correlates with resistance to challenge with virulent feline herpesvirus I (FHV-1), feline calicivirus (FCV), and feline parvovirus (FPV) in cats and to determine percentages of client-owned cats with serum antibodies to FHV-1, FCV, and FPV.Design-Prospective experimental study.Animals-72 laboratory-reared cats and 276 client-owned cats.Procedures-Laboratory-reared cats were vaccinated against FHV-1, FCV, and FPV, using 1 of 3 commercial vaccines, or maintained as unvaccinated controls. Between 9 and 36 months after vaccination, cats were challenged with virulent virus. Recombinant-antigen ELISA for detection of FHV-1-, FCV-, and FPV-specific antibodies were developed, and results were compared with results of hemagglutination inhibition (FPV) and virus neutralization (FHV-1 and FCV) assays and with resistance to viral challenge.Results-For vaccinated laboratory-reared cats, predictive values of positive results were 100% for the FPV and FCV ELISA and 90% for the FHV-1 ELISA. Results of the FHV-1, FCV, and FPV ELISA were positive for 195 (70.7%), 255 (92.4%), and 189 (68.5%), respectively, of the 276 client-owned cats.Conclusions and Clinical Relevance-Results suggest that for cats that have been vaccinated, detection of FHV-1-, FCV-, and FPV-specific antibodies is predictive of whether cats are susceptible to disease, regardless of vaccine type or vaccination interval. Because most client-owned cats had detectable serum antibodies suggestive of resistance to infection, use of arbitrary booster vaccination intervals is likely to lead to unnecessary vaccination of some cats.