Influence of Maternal-Fetal Histocompatibility and MHC Zygosity on Maternal Microchimerism1-猫眼课题宝

母胎组织相容性和 MHC 接合性对母体微嵌合的影响1

DOI：

发表时间：

2005

期刊：

Journal of Immunology

影响因子：

4.4

通讯作者：

中科院分区：

医学2区

文献类型：

作者：

J. Kaplan;S. Land

为了研究母胎组织相容性和母体微嵌合体之间的关系，我们开发了一种灵敏的新霉素抗性基因（neoR）定量PCR检测方法，并在小鼠模型系统中，使用neoR作为母体细胞的非遗传母体等位基因标记，检测和定量（neoR+/−）F1雌性与neoR−/−雄性交配的neoR−/− N2回交后代组织中的母体微嵌合体。使用这种方法，我们获得的证据表明，在所有断奶和成年小鼠的大脑，脾脏和胸腺嵌合母体细胞的存在。无论年龄或母胎组织相容性如何，脾脏中存在的嵌合母体细胞的数量与胸腺中的嵌合母体细胞的数量没有显著差异。在各年龄组中，与母亲MHC完全相同的小鼠脑组织中母体微嵌合体水平均高于淋巴组织，而与母亲MHC完全不同的小鼠脑组织中母体微嵌合体水平相似。在具有纯合同基因型和母体异基因型的小鼠的脑和淋巴组织中嵌合母体细胞的水平是相似的，并且倾向于高于具有组合的母胎异基因型或杂合同基因型的小鼠的组织特异性水平。因此，MHC纯合子后代比MHC杂合子后代具有更高水平的母体微嵌合体。我们的结论是，正常小鼠拥有少量的母细胞在脾脏，胸腺，大脑，可能是大多数其他组织，母胎组织相容性影响这些细胞的水平的机制有关的MHC接合性的后代。

To investigate the relationship between maternal-fetal histocompatibility and maternal microchimerism, we developed a sensitive quantitative PCR assay for the neomycin resistance gene (neoR), and, in a mouse model system, used neoR as a noninherited maternal allele marker of maternal cells to detect and quantitate maternal microchimerism in tissues of neoR−/− N2 backcross progeny of (neoR+/−)F1 females mated with neoR−/− males. Using this approach, we obtained evidence for the presence of chimeric maternal cells in the brain, spleen, and thymus of all weanling and adult mice so tested. The numbers of chimeric maternal cells present in the spleen did not differ significantly from those in the thymus regardless of age or maternal-fetal histocompatibility. At all ages, brain tissue had higher level of maternal microchimerism than lymphoid tissue in mice MHC identical with their mothers, but the levels were similar in mice MHC disparate with their mothers. The levels of chimeric maternal cells in both brain and lymphoid tissue of mice with homozygous syngenicity and maternal allogenicity were similar, and tended to be higher than tissue-specific levels in mice with either combined maternal-fetal allogenicity or heterozygous syngenicity. Thus, MHC homozygous progeny had higher levels of maternal microchimerism than MHC heterozygous progeny. We conclude that normal mice possess small numbers of maternal cells in spleen, thymus, brain, and probably most other tissues, and that maternal-fetal histocompatibility influences the levels of these cells by mechanisms related to MHC zygosity of the progeny.

A highly polymorphic microsatellite in the class II Eb gene allows tracing of major histocompatibility complex evolution in mouse.