Autoradiographic evidence of estrogen binding sites in nuclei of diethylstilbesterol induced hamster renal carcinomas.
Autoradiographic evidence of estrogen binding sites in nuclei of diethylstilbesterol induced hamster renal carcinomas.
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己烯雌醇诱导仓鼠肾癌细胞核中雌激素结合位点的放射自显影证据。
DOI：
10.1093/carcin/10.5.957
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发表时间：
1989
期刊：
影响因子：
4.7
通讯作者：
Pugh,TD
中科院分区：
文献类型：
--
作者：
Tse,J;Goldfarb,S;Pugh,TD
用放射自显影技术在3只金黄地鼠中发现了1个移植肾癌和5个二乙基己烯甾醇诱发的原发肾癌的雌激素结合部位。活体注射~3H-17β雌二醇后，放射性标记仅在肿瘤细胞核上增加；体视学分析显示，细胞核上还原的银粒浓度是细胞质的4.5-6.7倍；尽管雌二醇在管状排泄迅速，并在1小时内达到峰值，但正常细胞似乎没有与配体结合。这是在雌激素诱导的仓鼠肾癌中，首次发表的关于雌激素与细胞核结合的报道。
Estrogen binding sites were demonstrated by autoradiography in one transplantable and five primary diethylstilbesterol induced renal carcinomas in three hamsters. Radiolabelling, following thein vivoinjection of3H-17β estradiol, was increased only over the nuclei of tumor cells; stereologic analysis revealed a 4.5–6.7-times higher concentration of reduced silver grains over nuclei than cytoplasm of these cells; Despite rapid tubular excretion of estradiol which peaked in < 1 h, the normal cells did not appear to bind the ligand. This is the first published report documenting the preferentialin vivobinding of estrogen to nuclei of cells in estrogen induced hamster renal carcinomas.